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| Status |
Public on Mar 23, 2015 |
| Title |
Regulation of gene expression by glucose metabolism in mammary cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Reprogramming of cancer cell metabolism toward aerobic glycolysis, i.e. the Warburg effect, is a hallmark of cancer; according to current views, the rationale for selecting such energy-inefficient metabolism is the need to increase cellular biomass to sustain production of daughter cells and proliferation. In this view, metabolic reprogramming is considered as a simple phenotypic endpoint that occurs as a consequence of signal transduction mechanisms, including oncogene-driven nutrient uptake and metabolic rewiring. A newly emerging paradigm is instead that transcriptional networks and oncogenic signaling can also be regulated downstream of metabolic pathways, that assume causative roles in controlling cancer cell behavior, above and beyond their core biochemical function. To explore possible links between glucose metabolism and nuclear gene transcription we compared immortalized mammary epithelial cells (MCF10A) and metastatic breast cancer cells (MDA-MB-231) growing in high glucose or in the presence of a widely used inhibitor of glucose uptake / glucose metabolism, 2-deoxy-glucose (2DG).
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| Overall design |
MCF10A or MDA-MB-231 cells growing at low confluence in standard growth conditions were compared to cells incubated for 24 hours with 2-deoxy-glucose (2DG, 50mM) to inhibit glucose metabolism, and then harvested for total RNA extraction. 4 independent biological replicates were performed in parallel for each cell line and experimental condition.
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| Contributor(s) |
Dupont S, Bicciato S |
| Citation(s) |
25796446 |
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| Submission date |
Jul 09, 2014 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
silvio.bicciato@unipd.it
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| Phone |
+39-049-827-6108
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| Organization name |
University of Padova
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| Department |
Molecular Medicine
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| Street address |
via U. Bassi 59/b
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| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (16)
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| GSM1430997 |
MCF10A, biological replica D |
| GSM1430998 |
2DG treated MCF10A, biological replica A |
| GSM1430999 |
2DG treated MCF10A, biological replica B |
| GSM1431000 |
2DG treated MCF10A, biological replica C |
| GSM1431001 |
2DG treated MCF10A, biological replica D |
| GSM1431002 |
MDA-MB-231, biological replica A |
| GSM1431003 |
MDA-MB-231, biological replica B |
| GSM1431004 |
MDA-MB-231, biological replica C |
| GSM1431005 |
MDA-MB-231, biological replica D |
| GSM1431006 |
2DG treated MDA-MB-231, biological replica A |
| GSM1431007 |
2DG treated MDA-MB-231, biological replica B |
| GSM1431008 |
2DG treated MDA-MB-231, biological replica C |
| GSM1431009 |
2DG treated MDA-MB-231, biological replica D |
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| This SubSeries is part of SuperSeries: |
| GSE59232 |
Control of YAP/TAZ by glucose metabolism |
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| Relations |
| BioProject |
PRJNA254776 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE59228_RAW.tar |
78.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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