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Series GSE59228 Query DataSets for GSE59228
Status Public on Mar 23, 2015
Title Regulation of gene expression by glucose metabolism in mammary cell lines
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Reprogramming of cancer cell metabolism toward aerobic glycolysis, i.e. the Warburg effect, is a hallmark of cancer; according to current views, the rationale for selecting such energy-inefficient metabolism is the need to increase cellular biomass to sustain production of daughter cells and proliferation. In this view, metabolic reprogramming is considered as a simple phenotypic endpoint that occurs as a consequence of signal transduction mechanisms, including oncogene-driven nutrient uptake and metabolic rewiring. A newly emerging paradigm is instead that transcriptional networks and oncogenic signaling can also be regulated downstream of metabolic pathways, that assume causative roles in controlling cancer cell behavior, above and beyond their core biochemical function. To explore possible links between glucose metabolism and nuclear gene transcription we compared immortalized mammary epithelial cells (MCF10A) and metastatic breast cancer cells (MDA-MB-231) growing in high glucose or in the presence of a widely used inhibitor of glucose uptake / glucose metabolism, 2-deoxy-glucose (2DG).
Overall design MCF10A or MDA-MB-231 cells growing at low confluence in standard growth conditions were compared to cells incubated for 24 hours with 2-deoxy-glucose (2DG, 50mM) to inhibit glucose metabolism, and then harvested for total RNA extraction. 4 independent biological replicates were performed in parallel for each cell line and experimental condition.
Contributor(s) Dupont S, Bicciato S
Citation(s) 25796446
Submission date Jul 09, 2014
Last update date Mar 25, 2019
Contact name Silvio Bicciato
Phone +39-049-827-6108
Organization name University of Padova
Department Molecular Medicine
Street address via U. Bassi 59/b
City Padova
ZIP/Postal code 35131
Country Italy
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (16)
GSM1430994 MCF10A, biological replica A
GSM1430995 MCF10A, biological replica B
GSM1430996 MCF10A, biological replica C
This SubSeries is part of SuperSeries:
GSE59232 Control of YAP/TAZ by glucose metabolism
BioProject PRJNA254776

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59228_RAW.tar 78.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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