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Series GSE5470 Query DataSets for GSE5470
Status Public on Oct 16, 2006
Title Expression data from BY4743 and dbr1 yeast
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by genome tiling array
Summary Introns in pre-mRNAs must be spliced out prior to their translation. During splicing, introns are removed in the form of a lariat, in which the 5' end is linked to the 2' hydroxyl of an internal adenosine. Lariat degradation is initiated by an 2'-5' phosphodiester-specific RNA endonuclease which debranches these lariat RNAs to linear form. Deletion of the debranching enzyme is yeast results in the accumulation of lariat introns. We used this accumulation to identify spliced lariat introns on a genome-wide scale in S. cerevisiae using tiling microarrays.
Keywords: two sample comparison, 3 biological replicates
Overall design Total RNA from BY4743 and dbr1 yeast was harvested, labeled as double-stranded probes and hybridized to Affymetrix tiling microarrays. Signals enriched in the dbr1 strain were mapped to genomic coordinates to assess correspondence with known intron regions and identify novel spliced sequences.
Web link
Contributor(s) Fields S, Hesselberth J, Zhang Z
Citation(s) 17351133
Submission date Aug 07, 2006
Last update date Mar 16, 2012
Contact name Jay R. Hesselberth
Organization name University of Colorado School of Medicine
Department Biochemistry and Molecular Gentetics
Lab Jay Hesselberth
Street address 12801 E 17TH AVE
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
Platforms (1)
GPL4065 Affymetrix S. cerevisiae tiling 1.0R (Sc03b_MR_v03.bpmap)
Samples (6)
GSM125326 S. cerevisiae BY4743, biological rep1
GSM125327 S. cerevisiae BY4743, biological rep2
GSM125328 S. cerevisiae BY4743, biological rep3
BioProject PRJNA95983

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5470_RAW.tar 244.5 Mb (http)(custom) TAR (of CEL)

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