|
| Status |
Public on Sep 23, 2013 |
| Title |
Gene regulation by CBP-p300 in the lens placode - E10.5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Wild type or CBP/p300 fx/fx; Pax6-Cre-positive (CBP/p300-/-) surface ectoderm from E10.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified, pooled for each genotype and triplicate samples were reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6v2.0 bead arrays.
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| |
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| Overall design |
Three wild type and three knockout embryos were used. The RNA from embryos of the same genotype was purified and pooled and triplicate samples were amplified and used for microarray analysis.
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| Contributor(s) |
Beebe DC, Huang J, Liu Y |
| Citation(s) |
24038357 |
| |
| Submission date |
Jul 25, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
David Cy Beebe |
| E-mail(s) |
beebe@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Ophthalmology and Visual Science
|
| Lab |
Campus Box 8096
|
| Street address |
660 S. Euclid Ave.
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE49219 |
Histone posttranslational modifications and cell fate determination: Lens induction requires the lysine acetyltransferases CBP and p300 |
|
| Relations |
| BioProject |
PRJNA213305 |
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