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Series GSE44365 Query DataSets for GSE44365
Status Public on Jul 01, 2013
Title Epididymosomes convey different repertoires of miRNAs throughout the epididymis
Platform organism synthetic construct
Sample organism Bos taurus
Experiment type Non-coding RNA profiling by array
Summary Epididymosomes are small membrane vesicles (50-500nm) secreted by epididymal epithelial cells and involved in post-testicular sperm maturation. While their role in protein transfer to the sperm membrane is well acknowledged, we unveil here their capacity to vehicle microRNAs (miRNAs), which are potent regulators of post-transcriptional gene expression. Using a global microarray approach, we showed that epididymosomes providing from two discrete bovine epididymal regions (caput and cauda) possess distinct miRNA signatures. In addition, we established that miRNA repertoires contained in epididymosomes differ from those of their parent epithelial cells, suggesting that miRNA populations released from the cells may be selectively sorted. Binding of DilC12-labeled epididymosomes to primary cultured epididymal cells was measured by flow cytometry and indicated that epididymosomes from the median caput and their miRNA content may be incorporated into distal caput epithelial cells. Overall, these findings reveal that distinct miRNA repertoires are released into the intraluminal fluid in a region-specific manner and could be involved in a novel mechanism of intercellular communication throughout the epididymis via epididymosomes.
Overall design We determined the miRNA profiles of epididymosomes isolated by perfusion from caput and cauda regions of the epididymis and compared it with the miRNA content of epididymal epithelial cells from the same regions.
Contributor(s) Belleannée C, Calvo E, Sullivan R
Citation(s) 23803555
Submission date Feb 15, 2013
Last update date May 02, 2017
Contact name Ezequiel L Calvo
Organization name CRCHUL
Department Molecular Endocrinilogy
Lab Microarrays
Street address 2705 Boul. Laurier
City Quebec
State/province Quebec
ZIP/Postal code G1V 4G2
Country Canada
Platforms (1)
GPL8786 [miRNA-1] Affymetrix Multispecies miRNA-1 Array
Samples (12)
GSM1083958 Epididymal's epithelial cells from caput, replicate 1
GSM1083959 Epididymal's epithelial cells from caput, replicate 2
GSM1083960 Epididymal's epithelial cells from caput, replicate 3
BioProject PRJNA189565

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Supplementary file Size Download File type/resource
GSE44365_RAW.tar 2.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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