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Series GSE43834 Query DataSets for GSE43834
Status Public on Jun 20, 2013
Title A Stable Transcription Factor Complex Nucleated by Dimeric AML1-ETO Controls Leukaemogenesis
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary AML1-ETO, a fusion protein generated by the t(8;21) translocation in acute myeloid leukemia, is a transcription factor implicated in both gene repression and activation. We now show that, in leukemic cells, AML1-ETO resides in and functions through a stable protein complex (AETFC) that contains several hematopoietic transcription factors and cofactors. In conjunction with biochemical and leukemia pathological studies, the ChIP-seq and RNA-seq analyses of the AETFC components in leukemic cells reveal that these components stabilize the complex through multivalent interactions, provide multiple DNA-binding domains for diverse target genes, colocalize genome-wide, cooperatively regulate gene expression, and contribute to leukemogenesis.
Overall design RNA-seq analyses gene expression upon knockdown of each AETFC component, including AML1-ETO, HEB, E2A, LYL1, LDB1 and LMO2, and double-knockdown of HEB and E2A, in Kasumi-1 cells.
ChIP-seq analyses of four AETFC components, namely AML1-ETO, HEB, E2A and LMO2, in Kasumi-1 cells.
Contributor(s) Sun X, Jiang Y, Melnick AM, Roeder RG
Citation(s) 23812588
Submission date Jan 28, 2013
Last update date May 15, 2019
Contact name Yanwen Jiang
Organization name Weill Cornell Medical College
Street address 413 E. 69th St. BB1462
City New York
State/province NY
ZIP/Postal code 10021
Country USA
Platforms (2)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (17)
GSM1071852 shAML1-ETO
GSM1071853 shE2A
GSM1071854 shLYL1
BioProject PRJNA187930
SRA SRP018254

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Supplementary file Size Download File type/resource
GSE43834_RAW.tar 967.9 Mb (http)(custom) TAR (of TXT, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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