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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 13, 2012 |
| Title |
Microarray Analysis of a Familial Hypertrophic Cardiomyopathy Mouse Model Rescued by a Phospholamban Knockout |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Familial hypertrophic cardiomyopathy (FHC) is a disease characterized by ventricular hypertrophy, fibrosis, and aberrant systolic and/or diastolic function. Our laboratories have previously developed 2 mouse models that affect cardiac performance. One transgenic mouse model encodes an FHC-associated mutation in α-tropomyosin (Tm180) that displays severe cardiac hypertrophy with fibrosis and impaired physiological performance. The other model was a gene knockout of phospholamban (PLB), a regulator of calcium uptake in the sarcoplasmic reticulum of cardiomyocytes; the hearts of these mice exhibit hypercontractility with no pathological abnormalities. Previous work in our laboratories show that the hearts of mice that were genetically crossed between the Tm180 and PLB KO mice rescues the hypertrophic phenotype and improves their cardiac morphology and function.
We used microarrays to detail the global program of gene expression underlying cardiac remodeling and rescue of the hypertrophic cardiomyopathic phenotype and identified distinct classes of regulated genes during this process.
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| Overall design |
To understand the changes in gene expression that occur over time in these animal models (Tm180, PLB KO, Tm180/PLB KO and nontransgenic control mice), we conducted microarray analyses of left ventricular tissue at 4 and 12 months of age.
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| Contributor(s) |
Rajan S, Alves ML, Pena J, Jegga AG, Aronow BJ, Kranias EG, Wolska BM, Wieczorek DF |
| Citation(s) |
23800848 |
| Submission date |
Dec 12, 2012 |
| Last update date |
Mar 04, 2019 |
| Contact name |
sudarsan rajan |
| E-mail(s) |
sudarsan.rajan@cchmc.org
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| Organization name |
CCHMC
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| Street address |
3333 Burnet Ave
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| City |
Cincinnati |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (24)
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| GSM1052968 |
Tm180/PLB KO at 12 months, biological rep1 |
| GSM1052969 |
Tm180/PLB KO at 12 months, biological rep2 |
| GSM1052970 |
Tm180/PLB KO at 12 months, biological rep3 |
| GSM1052971 |
NTG at 12 months, biological rep1 |
| GSM1052972 |
NTG at 12 months, biological rep2 |
| GSM1052973 |
NTG at 12 months, biological rep3 |
| GSM1052974 |
Tm180 at 12 months, biological rep1 |
| GSM1052975 |
Tm180 at 12 months, biological rep2 |
| GSM1052976 |
Tm180 at 12 months, biological rep3 |
| GSM1052977 |
PLB KO at 4 months, biological rep1 |
| GSM1052978 |
PLB KO at 4 months, biological rep2 |
| GSM1052979 |
PLB KO at 4 months, biological rep3 |
| GSM1052980 |
Tm180/PLB KO at 4 months, biological rep1 |
| GSM1052981 |
Tm180/PLB KO at 4 months, biological rep2 |
| GSM1052982 |
Tm180/PLB KO at 4 months, biological rep3 |
| GSM1052983 |
NTG at 4 months, biological rep1 |
| GSM1052984 |
NTG at 4 months, biological rep2 |
| GSM1052985 |
NTG at 4 months, biological rep3 |
| GSM1052986 |
Tm180 at 4 months, biological rep1 |
| GSM1052987 |
Tm180 at 4 months, biological rep2 |
| GSM1052988 |
Tm180 at 4 months, biological rep3 |
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| Relations |
| BioProject |
PRJNA183715 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42892_RAW.tar |
102.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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