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Series GSE36048 Query DataSets for GSE36048
Status Public on Feb 24, 2012
Title A molecular mechanism for compartmentalization and silencing of chromatin domains at the nuclear lamina [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary A large fraction of the mammalian genome is organized into inactive chromosomal domains associated with the nuclear lamina. Using genomic repositioning assays we show that Lamina associated domains (LADs), spanning the developmentally regulated IgH and Cyp3a loci, contain transportable DNA regions that associate chromatin with the nuclear lamina and repress gene activity in fibroblasts.
Overall design We characterized DNA regions within LADs that are functionally capable of positioning chromatin domains at the inner nuclear membrane (INM) lamina. We mapped and characterized the IgH and other LADs in murine fibroblasts. We show that these murine LADs have a unique chromatin structure with discrete boundaries. We demonstrate DNA regions within LADs that are capable of directing the association of chromatin domains with the INM-lamina as well as the silencing of a co-integrated reporter gene.
Contributor(s) Zullo J, Epstein CB
Citation(s) 22726435
Submission date Feb 23, 2012
Last update date May 15, 2019
Contact name Joseph M Zullo
Organization name University of Chicago
Department MGCB
Lab Singh
Street address 929 E 57th Street
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (5)
GSM879920 H3K4me3_ChIPSeq
GSM879921 H3K36me3_ChIPSeq
GSM879922 H3K27me3_ChIPSeq
This SubSeries is part of SuperSeries:
GSE36049 A molecular mechanism for compartmentalization and silencing of chromatin domains at the nuclear lamina
SRA SRP011032
BioProject PRJNA155739

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Supplementary file Size Download File type/resource
GSE36048_RAW.tar 3.1 Gb (http)(custom) TAR (of BED, BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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