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| Status |
Public on Aug 21, 2005 |
| Title |
Influence of cyclical mechanical strain on global gene expression in human lamina cribrosa cells in vitro |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The mechanical effect of raised intraocular pressure is a recognised stimulus for optic neuropathy in primary open angle glaucoma (POAG). Characteristic extra-cellular matrix (ECM) remodelling accompanies axonal damage in the lamina cribrosa (LC) of the optic nerve head in POAG. Glial cells in the lamina cribrosa may play a role in this process but the precise cellular responses to mechanical forces in this region are unknown. The authors examined global gene expression profiles in lamina cribrosa cells exposed to cyclical mechanical stretch, with an emphasis on ECM genes.
Keywords: microarray, ECM, glaucoma, mechanical stretch, lamina cribrosa
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| Overall design |
n=3 stretch and static control experiments.
RNA pooled from each experiment and hybridised to individual experiment and control arrays.
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| Contributor(s) |
Kirwan RP, Fenerty CH, Crean' J, Wordinger RJ, Clark AF, O'Brien CJ |
| Citation(s) |
16205625 |
| |
| Submission date |
Aug 18, 2005 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Ruaidhri Patrick Kirwan |
| E-mail(s) |
ruaidhri.kirwan@ucd.ie
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| Organization name |
University College Dublin
|
| Department |
Conway Institute of Biomolecular and Biomedical Research & School of Medicine and Medical Science
|
| Lab |
Dr. Martin Leonard
|
| Street address |
Belfield
|
| City |
Dublin |
| State/province |
Leinster |
| ZIP/Postal code |
D4 |
| Country |
Ireland |
| |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (2) |
| GSM70917 |
static (control) experiment array |
| GSM70918 |
cyclical mechanical strain experiment (24 hours, 1 Hz , 15%) |
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| Relations |
| BioProject |
PRJNA93007 |
