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Series GSE29439 Query DataSets for GSE29439
Status Public on May 24, 2011
Title Global transcriptome response of recombinant protein xylanase producing E. coli in high cell density fed batch culture
Organism Escherichia coli
Experiment type Expression profiling by array
Summary Strong production of recombinant proteins interfere with cellular processes in many ways. The extent of the bacterial stress response is determined by the specific properties of the recombinant protein, and by the rates of transcription and translation. The consideration of bacterial stress and starvation responses is of crucial importance for the successful establishment of an industrial large scale bioprocess. Stress genes can be used as marker genes in order to monitor the fitness of industrial bacterial hosts during fermentation processes. For this purpose, here in our study we have applied transcriptome analysis for the description of general and specific stress and starvation responses of Escherichia coli. Producing recombinant protein (Xylanase) in high cell density fed batch culture.
Overall design Cells were grown in batch mode in TB media till an OD600 of 15. The specific growth rate of 0.3 h-1 was maintained by fed batch started at OD600 of 15. Cells were induced at an OD600 of 30 using 1 mM IPTG and the growth and product profile was monitored. Samples at different time points (2hrs, 4hrs, and 6hrs) were taken and frozen in liquid nitrogen quickly for transcriptome analysis.
Contributor(s) Mukherjee KJ, Gupta S, Sharma AK
Citation(s) 22018139
Submission date May 22, 2011
Last update date Mar 08, 2019
Contact name Krishna Jyoti Mukherjee
Phone 011-26704087
Organization name Jawaharlal Nehru University
Department School Of Biotechnology
Lab Bioprocess and Biosystems Enginerring Laboratory
Street address New Mehrauli Road
City New Delhi
State/province delhi
ZIP/Postal code 110067
Country India
Platforms (1)
GPL3154 [E_coli_2] Affymetrix E. coli Genome 2.0 Array
Samples (3)
GSM728703 specific growth rate 0.3h-1 2h
GSM728704 specific growth rate 0.3h-1 4h
GSM728731 specific growth rate 0.3h-1 6h
BioProject PRJNA141515

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29439_RAW.tar 3.1 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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