|
| Status |
Public on Jan 23, 2012 |
| Title |
Whole Transcriptome Data from Embryonic Mouse Limb-Buds |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by genome tiling array
|
| Summary |
Limb development is a powerful model to investigate transcriptional complexity during differentiation and development, as tissue types appear at various stages, with unique transcriptional signatures. So far, most studies have used conventional expression arrays, which can identify only a predetermined set of cDNAs. However there is growing evidence that most of the non-coding part of the genome is transcribed as well. We took advantage of genomic tiling arrays, which cover most of the mouse genome, to look at limb transcriptomes in an unbiased manner, by identifying all genic- and intergenic transcripts. We selected three developmental stages (E11.5 ; E13.5 ; E15.5), covering a large period of limb organogenesis, during which the limb evolves from a mostly homogenous mesenchymal cell population to more specialized tissue types such as cartilage, bone, muscle and skin.
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| |
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| Overall design |
Two biological replicates for each of three time points (E11.5, E13.5, E15.5).
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| |
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| Contributor(s) |
Gyurján I, Sonderegger B, Naef F, Duboule D |
| Citation(s) |
21801425 |
| |
| Submission date |
Feb 18, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Denis Duboule |
| Organization name |
EPFL
|
| Department |
ISREC
|
| Lab |
UPDUB
|
| Street address |
SV 2843 (Bâtiment SV)
|
| City |
Station 19 |
| State/province |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
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| Platforms (14)
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| GPL13194 |
[Mm35b_M01R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13195 |
[Mm35b_M02R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13196 |
[Mm35b_M03R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13197 |
[Mm35b_M04R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13198 |
[Mm35b_M05R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13199 |
[Mm35b_M06R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13200 |
[Mm35b_M07R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13201 |
[Mm35b_M08R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13202 |
[Mm35b_M09R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13203 |
[Mm35b_M10R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13204 |
[Mm35b_M11R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13205 |
[Mm35b_M12R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13206 |
[Mm35b_M13R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
| GPL13207 |
[Mm35b_M14R_v01] Affymetrix Mouse Tiling 1.1R Array Set |
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| Samples (84)
|
|
| Relations |
| BioProject |
PRJNA137243 |
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