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| Status |
Public on Mar 07, 2012 |
| Title |
Transcriptome analysis of a Bacillus subtilis mutant defective in the transcription termination factor Rho. |
| Platform organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Sample organism |
Bacillus subtilis |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
This analysis is part of the study GSE27219, The condition-dependent transcriptome of Bacillus subtilis 168. In this study, 120 transcription units where identified for which transcription did not terminate at any specific site, leading to mRNA extension over long distances with slowly decreasing signal intensity. In most cases, lack of termination and read-through generated antisense transcripts. These findings together with the lack of intrinsic terminators suggested that transcription termination of the 120 transcription units could be mediated by the transcription termination factor Rho. In order to investigate the impact of Rho-mediated termination, tiling array hybridizations using RNA samples of a B. subtilis rho-null mutant and its parental strain were performed.
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| Overall design |
B. subtilis 1012 wild-type and rho-mutant cells were grown in LB medium. Samples for tiling array analysis were taken during exponential growth phase. Hybridizations were performed in triplicate using RNA isolated from independent cultures.
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| Contributor(s) |
Mäder U, Bidnenko E, Nicolas P, Wiegert T |
| Citation(s) |
22383849 |
| |
| Submission date |
Feb 14, 2011 |
| Last update date |
Apr 07, 2014 |
| Contact name |
Ulrike Mäder |
| E-mail(s) |
ulrike.maeder@uni-greifswald.de
|
| Organization name |
University Medicine Greifswald
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| Department |
Functional Genomics
|
| Street address |
F.-L.-Jahn-Str. 15A
|
| City |
Greifswald |
| ZIP/Postal code |
D-17489 |
| Country |
Germany |
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| Platforms (1) |
| GPL13168 |
BaSysBio Bacillus subtilis T2 385K array [090325_CBS_DTU_Bsub_T2] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA137121 |
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