NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE27303 Query DataSets for GSE27303
Status Public on Mar 07, 2012
Title Transcriptome analysis of a Bacillus subtilis mutant defective in the transcription termination factor Rho.
Platform organism Bacillus subtilis subsp. subtilis str. 168
Sample organism Bacillus subtilis
Experiment type Expression profiling by genome tiling array
Summary This analysis is part of the study GSE27219, The condition-dependent transcriptome of Bacillus subtilis 168. In this study, 120 transcription units where identified for which transcription did not terminate at any specific site, leading to mRNA extension over long distances with slowly decreasing signal intensity. In most cases, lack of termination and read-through generated antisense transcripts. These findings together with the lack of intrinsic terminators suggested that transcription termination of the 120 transcription units could be mediated by the transcription termination factor Rho. In order to investigate the impact of Rho-mediated termination, tiling array hybridizations using RNA samples of a B. subtilis rho-null mutant and its parental strain were performed.
 
Overall design B. subtilis 1012 wild-type and rho-mutant cells were grown in LB medium. Samples for tiling array analysis were taken during exponential growth phase. Hybridizations were performed in triplicate using RNA isolated from independent cultures.
 
Contributor(s) Mäder U, Bidnenko E, Nicolas P, Wiegert T
Citation(s) 22383849
Submission date Feb 14, 2011
Last update date Apr 07, 2014
Contact name Ulrike Mäder
E-mail ulrike.maeder@uni-greifswald.de
Organization name University Medicine Greifswald
Department Functional Genomics
Street address F.-L.-Jahn-Str. 15A
City Greifswald
ZIP/Postal code D-17489
Country Germany
 
Platforms (1)
GPL13168 BaSysBio Bacillus subtilis T2 385K array [090325_CBS_DTU_Bsub_T2]
Samples (6)
GSM675099 wild type_replicate 1
GSM675100 wild type_replicate 2
GSM675101 wild type_replicate 3
Relations
BioProject PRJNA137121

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27303_RAW.tar 90.4 Mb (http)(custom) TAR (of PAIR)
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap