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Series GSE250282 Query DataSets for GSE250282
Status Public on Dec 19, 2023
Title sciMET-cap: High-throughput single-cell methylation analysis with a reduced sequencing burden
Organism Homo sapiens
Experiment type Other
Summary DNA methylation is a key component of the mammalian epigenome, playing a regulatory role in development, disease, and other processes. Robust, high-throughput single-cell DNA methylation assays are now possible (sciMET); however, the genome-wide nature of DNA methylation results in a high sequencing burden per cell. Here, we leverage target enrichment with sciMET to capture sufficient information per cell for cell type assignment using substantially fewer sequence reads (sciMET-cap). Sufficient off-target coverage further enables the production of near-complete methylomes for individual cell types. We characterize sciMET-cap on human PBMCs and brain (middle frontal gyrus).
 
Overall design Crypreserved commercially-obtained PBMCs were used for all data included in this data deposition
 
Contributor(s) Adey A
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Submission date Dec 15, 2023
Last update date Dec 19, 2023
Contact name Andrew Adey
E-mail(s) adey@ohsu.edu
Organization name Oregon Health & Science University
Department MMG
Lab Adey Lab
Street address 3181 S.W. Sam Jackson Park Rd, RHH, L103
City Portland
State/province OR
ZIP/Postal code 97239-3098
Country USA
 
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (5)
GSM7976773 sciMETcap on PBMCs, no capture control
GSM7976774 sciMETcap on PBMCs, standard blockers
GSM7976775 sciMETcap on PBMCs, custom blockers
Relations
BioProject PRJNA1053297

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE250282_processed_data.tar.gz 16.0 Gb (ftp)(http) TAR
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Raw data are available in SRA

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