|
| Status |
Public on Mar 07, 2024 |
| Title |
Single cell RNA sequencing of in vivo OVCAR8 tumor cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
CAR-T cell therapy has achieved remarkable clinical outcomes, yet the autologous nature of FDA-approved CAR-T products present significant challenges in manufacturing, cost, and patient selection. Therefore, there is a growing demand for off-the-shelf cell therapy. Here we introduce an ex vivo feeder-free culture to differentiate gene-engineered HSCs into allogeneic NKT cells, as well as their CAR-armed and IL-15-enhanced derivatives (Allo15CAR-NKT cells). In order to study the epigenetic regulation of the generated cells, we performed DNA methylation sequencing on both IL-15-enhanced and non-IL-15-engineered AlloCAR-NKT cells. Conventional CAR-T cells were included as a control.
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| Overall design |
Gene expression profiling of tumor cells in an in vivo study using an MM-FG human multiple myeloma xenograft NSG mouse model
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| |
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| Contributor(s) |
Li Y, Zhou Y, Yu J, Kim J, Li M, Yang L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 30, 2023 |
| Last update date |
Mar 08, 2024 |
| Contact name |
Lili Yang |
| E-mail(s) |
liliyang@g.ucla.edu
|
| Organization name |
UCLA Lili Yang Lab
|
| Street address |
610 Charles E. Young Dr. East, TLSB 3045
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1010942 |
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