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| Status |
Public on May 10, 2023 |
| Title |
Long-range genomic contacts and spatiotemporal chromatin landscape of human histone gene clusters at Histone Locus Bodies during the cell cycle in breast cancer [cHiC] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Cell cycle controlled mechanistic remodeling of chromatin architecture at HLBs as non-membranous phase-separated nuclear domains represents a novel dimension to regulation of cell proliferation. Therefore, we sought to resolve a gap in our understanding of how 3D higher-order genomic organization supports the activation of multiple clustered histone genes. Our findings are consistent model that the HINFP/NPAT complex controls the formation and dynamic remodeling of higher-order genomic organization of histone gene clusters at HLBs in early to late G1 phase to support production of histone mRNAs in S phase. that the two principal histone gene regulators HINFP and NPAT are located at anchor-points for chromatin loops reflecting their obligatory functions in remodeling the spatiotemporal genomic organization at HLBs during the G1 phase to accommodate histone gene expression in S phase.
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| Overall design |
cHiC in the MCF10DCIS cell, 2 biological replicates enriched for the histone locus body on chr6.
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| Contributor(s) |
Stein G, Boyd JR, Ghule P, Frietze S |
| Citation missing |
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| |
| Submission date |
Apr 10, 2023 |
| Last update date |
May 12, 2023 |
| Contact name |
Joseph R Boyd |
| Organization name |
University of Vermont
|
| Department |
Biomedical and Health Sciences
|
| Lab |
Frietze
|
| Street address |
University of Vermont
|
| City |
Burlington |
| State/province |
Vermont |
| ZIP/Postal code |
05405 |
| Country |
USA |
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| Platforms (1) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE229297 |
Long-range genomic contacts and spatiotemporal chromatin landscape of human histone gene clusters at Histone Locus Bodies during the cell cycle in breast cancer |
|
| Relations |
| BioProject |
PRJNA953981 |
