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| Status |
Public on Mar 07, 2024 |
| Title |
Transcriptomic and metabolic effect of immune checkpoint inhibitors on cellular components in renal cell tumor microenvironment and in Sunitinib resistant renal cell carcinoma [II] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Tumor microenvironmental cellular components like bone marrow cells stromal cells macrophages changes due to sunitinib resistance may affect their efficacy in advanced stage renal cell carcinomas. Immune checkpoint inhibitors (ICIs) have been approved as 2nd line therapy over acquired resistance. Each ICI uniquely affected different cellular components. Better understanding of transcriptomic, metabolomic or proteomic changes due to ICI treatment is necessary to development proper immunotherapy.
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| Overall design |
Stromal cells were treated with two PD1 inhibitors ICIs (P: Pembrolizumab, Niv: Nivolumab) for 24hr and RNA was extracted after each treatment. Differential gene expression analysis of each of the treated samples was performed using RNA-seq.
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| Contributor(s) |
Ghosh S, Sourbier C, Chou C, Wu W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 08, 2023 |
| Last update date |
Mar 07, 2024 |
| Contact name |
Carole Sourbier |
| Organization name |
FDA
|
| Department |
CBER
|
| Street address |
10903 New Hampshire Ave
|
| City |
Silver Spring |
| State/province |
MD |
| ZIP/Postal code |
20993 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
|
| GSM7089081 |
Stromal cell treated as Vehicle replicate_1 |
| GSM7089082 |
Stromal cell treated as Vehicle replicate_2 |
| GSM7089083 |
Stromal cell treated as Vehicle replicate_3 |
| GSM7089084 |
Stromal cell Avelumab treated replicate_1 |
| GSM7089085 |
Stromal cell Avelumab treated replicate_2 |
| GSM7089086 |
Stromal cell Avelumab treated replicate_3 |
| GSM7089087 |
Stromal cell Atezolizumab treated replicate_1 |
| GSM7089088 |
Stromal cell Atezolizumab treated replicate_2 |
| GSM7089089 |
Stromal cell Atezolizumab treated replicate_3 |
| GSM7089090 |
Stromal cell Durvalumab treated replicate_1 |
| GSM7089091 |
Stromal cell Durvalumab treated replicate_2 |
| GSM7089092 |
Stromal cell Durvalumab treated replicate_3 |
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| This SubSeries is part of SuperSeries: |
| GSE226969 |
Transcriptomic and metabolic effect of immune checkpoint inhibitors on cellular components in renal cell tumor microenvironment and in Sunitinib resistant renal cell carcinoma |
|
| Relations |
| BioProject |
PRJNA942329 |
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