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Series GSE21447 Query DataSets for GSE21447
Status Public on Dec 16, 2010
Title A global expression switch marks pachytene initiation during mouse male meiosis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Male spermatogenesis is an essential and complex developmental process with the purpose to gain totipotency and allow a whole new organism to develop upon fertilization. While single-gene based studies have provided insights into the mechanisms at work, global profiling is clearly required to deconvolute these processes. Here, by isolating highly enriched mouse meiotic cell populations, we report a comprehensive gene expression map of mammalian meiosis. Our data reveal in unique details the dynamic of fundamental expression patterns such as the global chromosome X inactivation and reactivation allowing to precisely dissect this unique process. It also reveals a rapid global expression switch at the initiation of the long pachytene stage reminiscent and coincident with the commitment to meiosis observed in budding yeast. Overall, this meiotic atlas provides an exhaustive blueprint and resource for deconvoluting mammalian gametogenesis and meiosis.
Overall design 10- to 16-week-old male C57BL/6 x DBA/2J F1 testis were used for sorting multiple meiotic fractions. These samples were used for subsequent RNA purification, labeling and hybridization to Affymetrix arrays. 1-4 biological replicates per fraction.
Contributor(s) Bois PR, Fallahi M, Getun IV, Wu ZK
Citation Fallahi M., Getun I.V., Wu Z.K., Bois P.R. A Global Expression Switch Marks Pachytene Initiation during Mouse Male Meiosis. Genes. 2010; 1(3):469-483.
Submission date Apr 21, 2010
Last update date Feb 11, 2019
Contact name Philippe R Bois
Organization name The Scripps Research Institute - Scripps Florida
Department Cancer Biology
Lab Genome Plasticity Laboratory
Street address 130 Scripps Way
City Jupiter
State/province FL
ZIP/Postal code 33458
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (20)
GSM536067 Sorted spermatogonia, biological rep1
GSM536068 Sorted spermatogonia, biological rep2
GSM536069 Sorted spermatogonia, biological rep3
BioProject PRJNA126335

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Supplementary file Size Download File type/resource
GSE21447_RAW.tar 71.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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