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| Status |
Public on Dec 16, 2010 |
| Title |
A global expression switch marks pachytene initiation during mouse male meiosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Male spermatogenesis is an essential and complex developmental process with the purpose to gain totipotency and allow a whole new organism to develop upon fertilization. While single-gene based studies have provided insights into the mechanisms at work, global profiling is clearly required to deconvolute these processes. Here, by isolating highly enriched mouse meiotic cell populations, we report a comprehensive gene expression map of mammalian meiosis. Our data reveal in unique details the dynamic of fundamental expression patterns such as the global chromosome X inactivation and reactivation allowing to precisely dissect this unique process. It also reveals a rapid global expression switch at the initiation of the long pachytene stage reminiscent and coincident with the commitment to meiosis observed in budding yeast. Overall, this meiotic atlas provides an exhaustive blueprint and resource for deconvoluting mammalian gametogenesis and meiosis.
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| Overall design |
10- to 16-week-old male C57BL/6 x DBA/2J F1 testis were used for sorting multiple meiotic fractions. These samples were used for subsequent RNA purification, labeling and hybridization to Affymetrix arrays. 1-4 biological replicates per fraction.
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| Contributor(s) |
Bois PR, Fallahi M, Getun IV, Wu ZK |
| Citation |
Fallahi M., Getun I.V., Wu Z.K., Bois P.R. A Global Expression Switch Marks Pachytene Initiation during Mouse Male Meiosis. Genes. 2010; 1(3):469-483. http://www.mdpi.com/2073-4425/1/3/469/
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| Submission date |
Apr 21, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Philippe R Bois |
| E-mail(s) |
pbois@scripps.edu
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| Organization name |
The Scripps Research Institute - Scripps Florida
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| Department |
Cancer Biology
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| Lab |
Genome Plasticity Laboratory
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| Street address |
130 Scripps Way
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| City |
Jupiter |
| State/province |
FL |
| ZIP/Postal code |
33458 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (20)
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| GSM536067 |
Sorted spermatogonia, biological rep1 |
| GSM536068 |
Sorted spermatogonia, biological rep2 |
| GSM536069 |
Sorted spermatogonia, biological rep3 |
| GSM536070 |
Sorted spermatogonia, biological rep4 |
| GSM536071 |
Sorted pre-Leptotene, biological rep1 |
| GSM536072 |
Sorted pre-Leptotene, biological rep2 |
| GSM536073 |
Sorted pre-Leptotene, biological rep3 |
| GSM536074 |
Sorted Leptotene/Zygotene, biological rep1 |
| GSM536075 |
Sorted Leptotene/Zygotene, biological rep2 |
| GSM536076 |
Sorted Leptotene/Zygotene, biological rep3 |
| GSM536077 |
Sorted Early-Pachytene |
| GSM536078 |
Sorted mid-Pachytene, biological rep1 |
| GSM536079 |
Sorted mid-Pachytene, biological rep2 |
| GSM536080 |
Sorted late-Pachytene |
| GSM536081 |
Sorted Diplotene, biological rep1 |
| GSM536082 |
Sorted Diplotene, biological rep2 |
| GSM536083 |
Sorted round spermatids, biological rep1 |
| GSM536084 |
Sorted round spermatids, biological rep2 |
| GSM536085 |
Sorted round spermatids, biological rep3 |
| GSM536086 |
Sorted round spermatids, biological rep4 |
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| Relations |
| BioProject |
PRJNA126335 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21447_RAW.tar |
71.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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