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| Status |
Public on Aug 11, 2023 |
| Title |
CUT&Tag analysis genome-wide maps of chromatin state in CTRLcas9 and SCARB2cas9 HepG2 cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the application of CUT&Tag sequencing technology for high-throughput profiling of c-Myc with core promoters of its target genes in mammalian cells. We performed CUT&Tag sequencing with antibody to total c-Myc with core promoters of its target genes in CTRLcas9 and SCARB2cas9 group. Inspection of multiple individual traces and global analyses showed that SCARB2cas9 globally reduced association of c-Myc with core promoters of its target genes.
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| Overall design |
HepG2 cells and HepG2 SCARB2Cas9 cells were harvested, and then the CUT&Tag assay was performed according to the manufacturer’s protocol using Hyperactive Universal CUT&Tag Assay Kit for Illumina. SCARB2cas9 reduced association of c-Myc with core promoters of its target genes.
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| Contributor(s) |
Li K, Jiang J, Wang F, Gao Y, Xue S |
| Citation(s) |
37739936 |
| Submission date |
Jul 07, 2022 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Yang Gao |
| E-mail(s) |
yxgyang@126.com
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| Phone |
18238837867
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| Organization name |
Chinese Academy of Medical Sciences & Peking Union Medical College
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| Department |
Institute of Medicinal Biotechnology
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| Street address |
Tiantan Xili 2
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| City |
Bei Jing |
| ZIP/Postal code |
100050 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE207673 |
Next-generation sequencing facilitates quantitative analysis of CON and PMB treated HepG2 cells and CreAlbMYC and CreAlbScarb2F/FMYC mice liver transcriptomes, and CUT&Tag analysis genome-wide maps of chromatin state in CTRLcas9 and SCARB2cas9 HepG2 cells |
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| Relations |
| BioProject |
PRJNA856663 |
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