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Series GSE207551 Query DataSets for GSE207551
Status Public on Jun 27, 2023
Title GATA2 mitotic bookmarking is required for definitive haematopoiesis [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In mitosis, most transcription factors detach from chromatin, but some are retained and bookmark genomic sites. Mitotic bookmarking has been implicated in lineage inheritance, pluripotency and reprogramming. However, the biological significance of this mechanism in vivo remains unclear. Here, we address mitotic retention of the hemogenic factors GATA2, GFI1B and FOS during haematopoietic specification. We show that GATA2 remains bound to chromatin throughout mitosis, in contrast to GFI1B and FOS, via C-terminal zinc finger-mediated DNA binding. GATA2 bookmarks a subset of its interphase targets that are co-enriched for RUNX1 and other regulators of definitive haematopoiesis. Remarkably, homozygous mice harbouring the cyclin B1 mitosis degradation domain upstream Gata2 partially phenocopy knockout mice. Degradation of GATA2 at mitotic exit abolishes definitive haematopoiesis at aorta-gonad-mesonephros, placenta and foetal liver, but does not impair yolk sac haematopoiesis. Our findings implicate GATA2-mediated mitotic bookmarking as critical for definitive haematopoiesis and highlight a dependency on bookmarkers for lineage commitment.
Overall design Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for GATA2 in asynchronous and mitotic K562 cells.
Contributor(s) Silvério-Alves R, Kurochkin I, Pereira F
Citation(s) 37580379
Submission date Jul 06, 2022
Last update date Sep 26, 2023
Contact name Ilia Kurochkin
Organization name Lund University
Street address BMC A12, Sölvegatan 17
City Lund
State/province Skane
ZIP/Postal code 221 84
Country Sweden
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM6294389 Asynchronous-K562-GATA2
GSM6294390 Mitotic-K562-GATA2
GSM6294391 Asynchronous-K562-input
BioProject PRJNA856146

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Supplementary file Size Download File type/resource
GSE207551_RAW.tar 664.7 Mb (http)(custom) TAR (of BED, BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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