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| Status |
Public on Sep 22, 2022 |
| Title |
Generation of a TPH2-EGFP reporter cell line for Purification and Monitoring of Human Serotonin neurons in vitro and in vivo |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Generation of serotonin neurons (SNs) from human pluripotent stem cells (hPSCs) provides a promising platform to explore the mechanisms of serotonin-associated neuropsychiatric disorders. However, neural differentiation always yields heterogeneous cell populations, making it difficult to identify and purify SNs in vitro or track them in vivo following transplantation. Herein, we generated a TPH2-EGFP reporter hPSC line with insertion of EGFP into the endogenous tryptophan hydroxylase 2 (TPH2) locus using CRISPR-Cas9-mediated gene editing technology. This TPH2-reporter, which faithfully indicated TPH2 expression during differentiation, enabled us to obtain purified SNs for subsequent transcriptional analysis and study of pharmacological responses to antidepressants. In addition, the reporter system showed strong EGFP expression to indicate SNs, which enabled us to explore in vitro and ex vivo electrophysiological properties of SNs. In conclusion, this TPH2-EGFP reporter cell line might be of great significance for studies on human SN-related development and differentiation, drug screening, disease modeling, and cell replacement therapies.
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| Overall design |
Comparative gene expression profiling of RNA-seq data from cells differentiated from hESCs to human serotonin neurons at three time points (Day0 (hESCs), Day21, Day42)
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| Contributor(s) |
Xu T, Lu J |
| Citation(s) |
36150384 |
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| Submission date |
Jun 17, 2022 |
| Last update date |
Oct 01, 2022 |
| Contact name |
Ting Xu |
| Organization name |
Tongji University
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| Street address |
1239 Siping Road
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| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (9)
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| GSM6251635 |
Differentiated cells, Day21, Bio rep 1 |
| GSM6251637 |
Differentiated cells, Day21, Bio rep 2 |
| GSM6251638 |
Differentiated cells, Day21, Bio rep 3 |
| GSM6251639 |
Differentiated cells, Day42, Bio rep 1 |
| GSM6251640 |
Differentiated cells, Day42, Bio rep 2 |
| GSM6251641 |
Differentiated cells, Day42, Bio rep 3 |
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| Relations |
| BioProject |
PRJNA850356 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE206341_4_genes_fpkm_expression.txt.gz |
6.8 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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