|
| Status |
Public on Nov 01, 2022 |
| Title |
Impact of COP1 silencing on hepatoma and hepatocyte transcriptomics |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Transfection experiments aimed at understanding the role of COP1 in hepatocytes and hepatoma models
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| |
|
| Overall design |
HuH-7 cells, HepG2 cells (hepatocyte-derived carcinoma cell line) or a lot of commercial primary hepatocytes (HMCPMS) from ThermoFisher Scientific were transfected for 96 h with 10 nM siRNA targeting either a RFWD2/COP1 or a control (NT1) siRNA. RNA was then harvested and the transcriptome analyzed by transcription array. Each file corresponds to a distinct biologic: the experiment was repeated on 2 -3 distinct occasions.
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| |
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| Contributor(s) |
Soubeyrand S, McPherson R |
| Citation missing |
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| Submission date |
Jun 14, 2022 |
| Last update date |
Nov 03, 2022 |
| Contact name |
Ruth McPherson |
| E-mail(s) |
rmcpherson@ottawaheart.ca
|
| Organization name |
UOHI
|
| Street address |
40 Ruskin
|
| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1Y4W7 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (17)
|
|
| Relations |
| BioProject |
PRJNA849227 |
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