|
| Status |
Public on Jul 31, 2023 |
| Title |
Sex shapes cell-type-specific transcriptional signatures of stress exposure in the mouse hypothalamus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Stress-related psychiatric disorders and the stress system show prominent differences between men and women. These sex differences are detectable at the transcriptional level but we lack cell-type specific information. Here, using single-cell transcriptomics, we identify cell-type-specific signatures of acute restraint stress in the paraventricular nucleus of the hypothalamus - a central hub of the stress response - in male and female mice. Further, we show that a history of chronic mild stress alters these acute stress signatures in a sex-specific way. Using this dataset, we identified oligodendrocytes as a major target for sex-specific effects of stress. This dataset provides the first molecular resource for an in-depth dissection of the interplay between cell types and sex on the mechanisms of the stress response, offering the transcriptomes of thousands of individual cells.
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| |
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| Overall design |
Single-cell RNA-sequencing of male and female mice without (controls) or after stress exposure (acute restraint stress (ARS) or acute restraint stress after chronic mild stress (CMS ARS))
|
| Web link |
https://www.cell.com/cell-reports/fulltext/S2211-1247(23)00885-9
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| |
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| Contributor(s) |
Brivio E, Lopez J, Chen A |
| Citation(s) |
37516966 |
| |
| Submission date |
Apr 19, 2022 |
| Last update date |
Aug 01, 2023 |
| Contact name |
Alon Chen |
| E-mail(s) |
alon.chen@weizmann.ac.il
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Brain Sciences
|
| Street address |
Arison Neurobiology Building
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA828111 |
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