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| Status |
Public on Nov 16, 2021 |
| Title |
Chromatin isolation by RNA purification sequencing of LINC02015 in human aortic smooth muscle cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
This study was designed to explore the role of LINC02015 in regulating biological behaviors of vascular smooth muscle cells (VSMCs) and investigate the interacting targets of LINC02015 with high-throughput techniques and bioinformatics analysis. Chromatin isolation by RNA purification (ChIRP) sequencing was applied to explore the interaction relationship among LncRNA, RNA-binding protein and DNA. Localization of the active binding sites was specified by high-throughput sequencing with STAR2. Peak calling analysis was applied to spot enriched gene peaks with MACS.
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| Overall design |
Examination of LINC02015 binding sites in human aortic smooth muscle cells
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| Contributor(s) |
Ji Q |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 13, 2021 |
| Last update date |
Nov 16, 2021 |
| Contact name |
Qiang Ji |
| Organization name |
Zhongshan Hospital Fudan University
|
| Street address |
180 Fenglin Rd.
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA780173 |
| SRA |
SRP345942 |
