|
| Status |
Public on Dec 31, 2021 |
| Title |
Regulation of Chromatin Looping by CTCF and Cohesin |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Other
|
| Summary |
As part of a study to understand the dynamics of chromatin looping and its regulation by CTCF and cohesin, we have determined the genome-wide binding profiles of CTCF and cohesin (Smc1a subunit) in a genome-edited mouse Embryonic Stem Cell (mESC) line referred to as clone C65. We have also performed 3D genome mapping using Micro-C in another genome-edited mESC line referred to as clone C36.
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| |
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| Overall design |
We performed Micro-C in the mESC clone C36 (5 replicates) and ChIP-seq in the mESC clone C65 (input, normal rabbit IgG, rabbit anti-CTCF and rabbit anti-Smc1a antibodies, single replicates).
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| |
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| Contributor(s) |
Hansen AS, Hsieh TS, Cattoglio C, Gabriele M, Jha A, Brandao H, Mirny L, Grosse-Holz SB, Zechner C, Dailey GM |
| Citation(s) |
35420890 |
| |
| Submission date |
Nov 03, 2021 |
| Last update date |
May 22, 2023 |
| Contact name |
Xavier Darzacq |
| E-mail(s) |
darzacq@berkeley.edu
|
| Phone |
510-642-0884
|
| Organization name |
University of California, Berkeley
|
| Department |
Molecular and Cell Biology
|
| Lab |
Darzacq Lab
|
| Street address |
475D Li Ka Shing Center
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA777586 |
| SRA |
SRP344404 |
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