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Status |
Public on Dec 31, 2021 |
Title |
Regulation of Chromatin Looping by CTCF and Cohesin |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing Other
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Summary |
As part of a study to understand the dynamics of chromatin looping and its regulation by CTCF and cohesin, we have determined the genome-wide binding profiles of CTCF and cohesin (Smc1a subunit) in a genome-edited mouse Embryonic Stem Cell (mESC) line referred to as clone C65. We have also performed 3D genome mapping using Micro-C in another genome-edited mESC line referred to as clone C36.
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Overall design |
We performed Micro-C in the mESC clone C36 (5 replicates) and ChIP-seq in the mESC clone C65 (input, normal rabbit IgG, rabbit anti-CTCF and rabbit anti-Smc1a antibodies, single replicates).
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Contributor(s) |
Hansen AS, Hsieh TS, Cattoglio C, Gabriele M, Jha A, Brandao H, Mirny L, Grosse-Holz SB, Zechner C, Dailey GM |
Citation(s) |
35420890 |
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Submission date |
Nov 03, 2021 |
Last update date |
May 22, 2023 |
Contact name |
Xavier Darzacq |
E-mail(s) |
darzacq@berkeley.edu
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Phone |
510-642-0884
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Organization name |
University of California, Berkeley
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Department |
Molecular and Cell Biology
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Lab |
Darzacq Lab
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Street address |
475D Li Ka Shing Center
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City |
Berkeley |
State/province |
CA |
ZIP/Postal code |
94720 |
Country |
USA |
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Platforms (1) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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Samples (9)
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Relations |
BioProject |
PRJNA777586 |
SRA |
SRP344404 |