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Series GSE186509 Query DataSets for GSE186509
Status Public on Nov 23, 2021
Title Human intestinal bitter taste receptors regulate innate immune responses and metabolic regulators in obesity
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary An expression profiling was conducted to analyse the effect of the bitter tasting compounds denatonium benzoate (DB) or aloin on the transcriptome of human jejunal crypts compared to DMEM-treated crypts. These two bitter compounds are agonists for the human bitter taste receptor TAS2R43. We took advantage of a deletion polymorphism for TAS2R43, that exists in about 33% of the population to compare the effects of the TAS2R43 agonists in obese subjects that express (TAS2R43(+)) or do not express (TAS2R43(-)) TAS2R43. Primary jejunal crypts from lean (multi-organ donors) or obese (RYGB surgery) subjects were cultured for 24 hours and treated for 4 hours with either DMEM (control) or 1 mM DB or 30 µM aloin. In total 48 mRNA samples were subjected to RNAseq analysis.
 
Overall design 6 groups of human jejunal crypts of obese subjects: 1) TAS2R43+, DMEM treatment (n=8), 2) TAS2R43+, 1 mM denatonium benzoate (DB) (n=7), 3) TAS2R43+, 30µM aloin (n=6), 4) TAS2R43-, DMEM treatment (n=5), 5) TAS2R43-, 1 mM DB (n=6), 6) TAS2R43-, 30 µM aloin (n=4) 3 groups of human jejunal crypts of lean subjects with a TAS2R43+ genotype: 1) DMEM treatment (n=4), 1 mM DB (n=4), 5) 30 µM aloin (n=4)
 
Contributor(s) Liszt KI
Citation(s) 34784295
Submission date Oct 25, 2021
Last update date Nov 25, 2021
Contact name Kathrin Ingrid Liszt
E-mail(s) kathrin.liszt@ccri.at
Organization name KU Leuven
Lab Gut Peptide Research Lab
Street address O&N1 Herestraat 49 – bus 701
City Leuven
ZIP/Postal code 3000
Country Belgium
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (48)
GSM5654906 GC086067
GSM5654907 GC086068
GSM5654908 GC086069
Relations
BioProject PRJNA774256
SRA SRP342999

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE186509_RAW.tar 8.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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