|
| Status |
Public on Jan 25, 2023 |
| Title |
Small RNA next genration sequencing of miRNAs in Withaferin A treated MDA-MB-231 cells. |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
In this study, we reporte the altered miRNAs expression in the human triple negative breast cancer cell line (MDA-MB-231) in response to withaferin A at IC50 (2 µM) concentration.
|
| |
|
| Overall design |
Withaferin A is a major pharmacological active constituent of Withania somnifera (Solanaceae) plant. In this study we were aimed to identify the diffentially expressed known/novel miRNAs using next generation sequencing (NGS) in withaferin A treated MDA-MB-231 cells. For this, Human breast TNBC (MDA-MB-231) cells were cultured in the Dulbecco’s Modified Eagle Medium (DMEM) media supplemented with 10% (v/v) heat-inactivated FBS, 100 U/ml of penicillin, and 100 mg/ml of streptomycin and maintained in 5% CO2 at 37 degre celcius in a humidified atmosphere of 95% air in CO2 incubator. A total of 1x105 MDA-MB-231 cells were seeded in 35mm treated culture dishes containing 1ml of DMEM culture media and sub-cultured at every 3–4 days. The cultured MDA-MB-231 cells were treated with WA (2 µM) for 24 h as experimental group and without WA treated MDA-MB-231 cells were taken as control group. Three samples were assigned for each group.
|
| |
|
| Contributor(s) |
Kumar S |
| Citation(s) |
36676955 |
| |
| Submission date |
Sep 03, 2021 |
| Last update date |
Jan 26, 2023 |
| Contact name |
Shashank Kumar |
| E-mail(s) |
shashankbiochemau@gmail.com
|
| Organization name |
CENTRAL UNIVERSITY OF PUNJAB
|
| Department |
Biochemistry
|
| Lab |
Molecular Signaling & Drug Discovery Laboratory
|
| Street address |
VPO Guddha
|
| City |
Bathinda |
| State/province |
Punjab |
| ZIP/Postal code |
151401 |
| Country |
India |
| |
|
| Platforms (1) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA760502 |
| SRA |
SRP335568 |
Less...
More...