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Series GSE183395 Query DataSets for GSE183395
Status Public on Jan 25, 2023
Title Small RNA next genration sequencing of miRNAs in Withaferin A treated MDA-MB-231 cells.
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary In this study, we reporte the altered miRNAs expression in the human triple negative breast cancer cell line (MDA-MB-231) in response to withaferin A at IC50 (2 µM) concentration.
Overall design Withaferin A is a major pharmacological active constituent of Withania somnifera (Solanaceae) plant. In this study we were aimed to identify the diffentially expressed known/novel miRNAs using next generation sequencing (NGS) in withaferin A treated MDA-MB-231 cells. For this, Human breast TNBC (MDA-MB-231) cells were cultured in the Dulbecco’s Modified Eagle Medium (DMEM) media supplemented with 10% (v/v) heat-inactivated FBS, 100 U/ml of penicillin, and 100 mg/ml of streptomycin and maintained in 5% CO2 at 37 degre celcius in a humidified atmosphere of 95% air in CO2 incubator. A total of 1x105 MDA-MB-231 cells were seeded in 35mm treated culture dishes containing 1ml of DMEM culture media and sub-cultured at every 3–4 days. The cultured MDA-MB-231 cells were treated with WA (2 µM) for 24 h as experimental group and without WA treated MDA-MB-231 cells were taken as control group. Three samples were assigned for each group.
Contributor(s) Kumar S
Citation(s) 36676955
Submission date Sep 03, 2021
Last update date Jan 26, 2023
Contact name Shashank Kumar
Department Biochemistry
Lab Molecular Signaling & Drug Discovery Laboratory
Street address VPO Guddha
City Bathinda
State/province Punjab
ZIP/Postal code 151401
Country India
Platforms (1)
GPL21697 NextSeq 550 (Homo sapiens)
Samples (6)
GSM5556752 SKWANT-repl1
GSM5556753 SKWANT-repl2
GSM5556754 SKWANT-repl3
BioProject PRJNA760502
SRA SRP335568

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE183395_RAW.tar 340.0 Kb (http)(custom) TAR (of XLSX)
GSE183395_Read_count_expression_matrix.csv.gz 30.2 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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