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Series GSE181257 Query DataSets for GSE181257
Status Public on Mar 02, 2023
Title Functionally distinct promoter classes initiate transcription via different mechanisms [PRO-Seq]
Organism Drosophila melanogaster
Experiment type Other
Summary To identify the functional contribution to transcription of various promoter-bound proteins we have endogenously tagged select proteins with an auxin-inducible degron and measured nascent transcription with PRO-seq 6 hours after their depletion
Overall design Endogenously tagged AID cell lines were created using CRISPR/Cas9 in Drosophila S2 cells constitutively expressing OsTir ligase. PRO-seq was performed 3, 6 or 12 hours after auxin addition
Contributor(s) Stark A, Serebreni L, Pleyer L
Citation(s) 37013908
Submission date Jul 31, 2021
Last update date Jun 01, 2023
Contact name Alexander Stark
Organization name The Research Institute of Molecular Pathology (IMP)
Lab Stark Lab
Street address Campus-Vienna-Biocenter 1
City Vienna
ZIP/Postal code 1030
Country Austria
Platforms (2)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
GPL22106 NextSeq 550 (Drosophila melanogaster)
Samples (22)
GSM5493639 TFIIA control 1
GSM5493640 TFIIA control 2
GSM5493641 TFIIA 6 hour auxin 1
BioProject PRJNA751383
SRA SRP330769

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Supplementary file Size Download File type/resource
GSE181257_processed_data.tar.gz 338.5 Mb (ftp)(http) TAR
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Raw data are available in SRA
Processed data are available on Series record

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