|
| Status |
Public on Mar 02, 2023 |
| Title |
Functionally distinct promoter classes initiate transcription via different mechanisms [PRO-Seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
|
| Summary |
To identify the functional contribution to transcription of various promoter-bound proteins we have endogenously tagged select proteins with an auxin-inducible degron and measured nascent transcription with PRO-seq 6 hours after their depletion
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| |
|
| Overall design |
Endogenously tagged AID cell lines were created using CRISPR/Cas9 in Drosophila S2 cells constitutively expressing OsTir ligase. PRO-seq was performed 3, 6 or 12 hours after auxin addition
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| |
|
| Contributor(s) |
Stark A, Serebreni L, Pleyer L |
| Citation(s) |
37013908 |
| |
| Submission date |
Jul 31, 2021 |
| Last update date |
Jun 01, 2023 |
| Contact name |
Alexander Stark |
| E-mail(s) |
stark@starklab.org
|
| Organization name |
The Research Institute of Molecular Pathology (IMP)
|
| Lab |
Stark Lab
|
| Street address |
Campus-Vienna-Biocenter 1
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
|
| Platforms (2) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
| GPL22106 |
NextSeq 550 (Drosophila melanogaster) |
|
| Samples (22)
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|
| Relations |
| BioProject |
PRJNA751383 |
| SRA |
SRP330769 |
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