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| Status |
Public on Jul 29, 2024 |
| Title |
Small RNA next generation sequencing of miRNAs in MCF7 cells derived mammosphere treated with withaferin A |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
In this study, we reporte the altered miRNAs expression in the human breast cancer cell line (MCF7) derived mammosphere in response to withaferin A IC50 concentrations (2 µM)..
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| Overall design |
Withaferin A is a major pharmacological active constituent of Withania somnifera (Solanaceae). In this study we were aimed to identify the diffentially expressed known/novel miRNAs using next generation sequencing (NGS) in withaferin A treated MCF7 derived mammosphere. For this, MCF7 cells were initially treated with withaferin A with 2 µM concentration (experimental groups) and without treated MCF7 cells (the control group) were allowed to form spheres and incubated at 37 ºC under a 95% humid environment in an incubator having 5% CO2 for five days. Three samples were assigned for each group.
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| Citation missing |
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| Submission date |
Jul 29, 2021 |
| Last update date |
Jul 29, 2024 |
| Contact name |
Shashank Kumar |
| E-mail(s) |
shashankbiochemau@gmail.com
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| Organization name |
CENTRAL UNIVERSITY OF PUNJAB
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| Department |
Biochemistry
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| Lab |
Molecular Signaling & Drug Discovery Laboratory
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| Street address |
VPO Guddha
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| City |
Bathinda |
| State/province |
Punjab |
| ZIP/Postal code |
151401 |
| Country |
India |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA750717 |
| SRA |
SRP330431 |
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