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Series GSE18025 Query DataSets for GSE18025
Status Public on Sep 12, 2009
Title Fibrinogen-reactive T cells transfer disease to naïve mice
Platform organisms Homo sapiens; Mus musculus
Sample organism Mus musculus
Experiment type Protein profiling by protein array
Summary Splenocytes harvested from mice with FIA were cultured in vitro and stimulated with 0.01 mg/ml fibrinogen for 3 days. Enriched T cells were transferred into 6-week-old naïve SJL mice, which developed visible signs of arthritis within 2 weeks. Synovial array profiling of plasma from the arthritic recipient mice demonstrated autoreactive B cell responses against peptides representing native fibrinogen, and further spreading of the responses to target collagen type V, cartilage gp39, and citrullinated vimentin.
Overall design Custom-spotted protein slides were with plasma samples from individual mice. Four slides were probed with plasma derived from naïve mice and five slides were probed with plasma derived from mice injected with FIA T cells.
Contributor(s) Ho PP, Lee LY, Zhao X, Tomooka BH, Paniagua RT, Sharpe O, BenBarak MJ, Hueber W, Chandra PE, Steinman L, Robinson WH
Citation(s) 19949094
Submission date Sep 09, 2009
Last update date Mar 21, 2012
Contact name William Robinson
Phone 6508491207
Fax 6508491208
Organization name Stanford University
Street address 269 Campus Dr
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL9165 Synovial protein array 0.5K v2.0
Samples (9)
GSM450723 Naive - 1
GSM450724 Naive - 2
GSM450725 Naive - 3
This SubSeries is part of SuperSeries:
GSE18073 Mice with fibrinogen-induced arthritis (FIA)
BioProject PRJNA123465

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18025_RAW.tar 1010.0 Kb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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