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| Status |
Public on Aug 06, 2009 |
| Title |
Photoperiod regulation of grape bud dormancy |
| Platform organism |
Vitis vinifera |
| Sample organisms |
Vitis riparia; Vitis hybrid cultivar |
| Experiment type |
Expression profiling by array
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| Summary |
Bud endodormancy induction response of two genotypes (‘Seyval’ a hybrid white wine grape and V. riparia, PI588259 a native north american species) was compared under long (15h) and short (13h) photoperiod. Three separate replicates (5 plants/replicate) were treated to generate paradormant (LD) and same aged endodormancy-induced (SD) buds for transcriptomic, proteomic and metabolomic analysis. Potted, spur-pruned two to six-year-old vines were removed from cold storage (Seyval 3-19-07; V. riparia 3/26/07) and grown under a LD (15 h) at 25/20 + 3C day/night temperatures (D/N). When vines reached 12-15 nodes (3-25-07) they were randomized into LD or SD treatments with 25/20 + 3C D/N in climate controlled greenhouses with automated photoperiod system (VRE Greenhouse Systems). Three replications (5 vines/replication) were harvested between 5/07-6/07 and then again in 5/08-6/08 for a total of six replications. All treatments are repeated at the same time every year and harvested at the same time of day each year to minimize biological noise. At 1, 3, 7, 14, 21, 28 and 42 days of LD and SD treatment, buds were harvested from nodes 3 to 12 of each separate replicate, immediately frozen in liquid nitrogen, and placed at -80C for future RNA, protein and metabolite extraction. These time points encompass early reversible phases as well as key time points during transition to irreversible endodormancy development. After photoperiod treatments and bud harvests, all pruned vines were returned to LD and monitored for bud endodormancy. The endodormant vines were identified after 28 days and moved to cold storage. The nondormant vines were allowed to grow again and induced into dormancy at a later date. Acknowledgement:This study was funded by NSF Grant DBI0604755 and funds from the South Dakota Agriculture Experiment Station. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Anne Fennell. The equivalent experiment is VV10 at PLEXdb.]
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| Overall design |
84 Samples of two genotypes compared under long and short photoperiod, 3 replicates each.
Seyval (SV, Seyve Villard 5-276) : LD, 15h : 1 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : LD, 15h : 3 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : LD, 15h : 7 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : LD, 15h : 14 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : LD, 15h : 21 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : LD, 15h : 28 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : LD, 15h : 42 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : SD, 13h : 1 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : SD, 13h : 3 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : SD, 13h : 7 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : SD, 13h : 14 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : SD, 13h : 21 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : SD, 13h : 28 days (3-replications)
Seyval (SV, Seyve Villard 5-276) : SD, 13h : 42 days (3-replications)
V. riparia (VR, PI588259) : LD, 15h : 1 days (3-replications)
V. riparia (VR, PI588259) : LD, 15h : 3 days (3-replications)
V. riparia (VR, PI588259) : LD, 15h : 7 days (3-replications)
V. riparia (VR, PI588259) : LD, 15h : 14 days (3-replications)
V. riparia (VR, PI588259) : LD, 15h : 21 days (3-replications)
V. riparia (VR, PI588259) : LD, 15h : 28 days (3-replications)
V. riparia (VR, PI588259) : LD, 15h : 42 days (3-replications)
V. riparia (VR, PI588259) : SD, 13h : 1 days (3-replications)
V. riparia (VR, PI588259) : SD, 13h : 3 days (3-replications)
V. riparia (VR, PI588259) : SD, 13h : 7 days (3-replications)
V. riparia (VR, PI588259) : SD, 13h : 14 days (3-replications)
V. riparia (VR, PI588259) : SD, 13h : 21 days (3-replications)
V. riparia (VR, PI588259) : SD, 13h : 28 days (3-replications)
V. riparia (VR, PI588259) : SD, 13h : 42 days (3-replications)
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| Web link |
http://www.plexdb.org/modules/PD_browse/experiment_browser.php?experiment=VV10
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| Contributor(s) |
Fennell A |
| Citation(s) |
20151315 |
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| Submission date |
Aug 04, 2009 |
| Last update date |
Jun 22, 2017 |
| Contact name |
PLEXdb Curator, Sudhansu Dash |
| Organization name |
Iowa State University
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| Department |
Virtual Reality Application Center
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| Lab |
PLEXdb
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| Street address |
1023 Crop Genomes Informatics Lab (Iowa State University)
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| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
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| Platforms (1) |
| GPL1320 |
[Vitis_Vinifera] Affymetrix Vitis vinifera (Grape) Genome Array |
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| Samples (84)
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| GSM436346 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 1 days - rep1 |
| GSM436347 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 1 days - rep2 |
| GSM436348 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 1 days - rep3 |
| GSM436349 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 3 days - rep1 |
| GSM436350 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 3 days - rep2 |
| GSM436351 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 3 days - rep3 |
| GSM436352 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 7 days - rep1 |
| GSM436353 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 7 days - rep2 |
| GSM436354 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 7 days - rep3 |
| GSM436355 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 14 days - rep1 |
| GSM436356 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 14 days - rep2 |
| GSM436357 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 14 days - rep3 |
| GSM436358 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 21 days - rep1 |
| GSM436359 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 21 days - rep2 |
| GSM436360 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 21 days - rep3 |
| GSM436361 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 28 days - rep1 |
| GSM436362 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 28 days - rep2 |
| GSM436363 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 28 days - rep3 |
| GSM436364 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 42 days - rep1 |
| GSM436365 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 42 days - rep2 |
| GSM436366 |
Seyval (SV, Seyve Villard 5-276) LD, 15h 42 days - rep3 |
| GSM436367 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 1 days - rep1 |
| GSM436368 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 1 days - rep2 |
| GSM436369 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 1 days - rep3 |
| GSM436370 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 3 days - rep1 |
| GSM436371 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 3 days - rep2 |
| GSM436372 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 3 days - rep3 |
| GSM436373 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 7 days - rep1 |
| GSM436374 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 7 days - rep2 |
| GSM436375 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 7 days - rep3 |
| GSM436376 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 14 days - rep1 |
| GSM436377 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 14 days - rep2 |
| GSM436378 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 14 days - rep3 |
| GSM436379 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 21 days - rep1 |
| GSM436380 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 21 days - rep2 |
| GSM436381 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 21 days - rep3 |
| GSM436382 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 28 days - rep1 |
| GSM436383 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 28 days - rep2 |
| GSM436384 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 28 days - rep3 |
| GSM436385 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 42 days - rep1 |
| GSM436386 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 42 days - rep2 |
| GSM436387 |
Seyval (SV, Seyve Villard 5-276) SD, 13h 42 days - rep3 |
| GSM436388 |
V. riparia (VR, PI588259) LD, 15h 1 days - rep1 |
| GSM436389 |
V. riparia (VR, PI588259) LD, 15h 1 days - rep2 |
| GSM436390 |
V. riparia (VR, PI588259) LD, 15h 1 days - rep3 |
| GSM436391 |
V. riparia (VR, PI588259) LD, 15h 3 days - rep1 |
| GSM436392 |
V. riparia (VR, PI588259) LD, 15h 3 days - rep2 |
| GSM436393 |
V. riparia (VR, PI588259) LD, 15h 3 days - rep3 |
| GSM436394 |
V. riparia (VR, PI588259) LD, 15h 7 days - rep1 |
| GSM436395 |
V. riparia (VR, PI588259) LD, 15h 7 days - rep2 |
| GSM436396 |
V. riparia (VR, PI588259) LD, 15h 7 days - rep3 |
| GSM436397 |
V. riparia (VR, PI588259) LD, 15h 14 days - rep1 |
| GSM436398 |
V. riparia (VR, PI588259) LD, 15h 14 days - rep2 |
| GSM436399 |
V. riparia (VR, PI588259) LD, 15h 14 days - rep3 |
| GSM436400 |
V. riparia (VR, PI588259) LD, 15h 21 days - rep1 |
| GSM436401 |
V. riparia (VR, PI588259) LD, 15h 21 days - rep2 |
| GSM436402 |
V. riparia (VR, PI588259) LD, 15h 21 days - rep3 |
| GSM436403 |
V. riparia (VR, PI588259) LD, 15h 28 days - rep1 |
| GSM436404 |
V. riparia (VR, PI588259) LD, 15h 28 days - rep2 |
| GSM436405 |
V. riparia (VR, PI588259) LD, 15h 28 days - rep3 |
| GSM436406 |
V. riparia (VR, PI588259) LD, 15h 42 days - rep1 |
| GSM436407 |
V. riparia (VR, PI588259) LD, 15h 42 days - rep2 |
| GSM436408 |
V. riparia (VR, PI588259) LD, 15h 42 days - rep3 |
| GSM436409 |
V. riparia (VR, PI588259) SD, 13h 1 days - rep1 |
| GSM436410 |
V. riparia (VR, PI588259) SD, 13h 1 days - rep2 |
| GSM436411 |
V. riparia (VR, PI588259) SD, 13h 1 days - rep3 |
| GSM436412 |
V. riparia (VR, PI588259) SD, 13h 3 days - rep1 |
| GSM436413 |
V. riparia (VR, PI588259) SD, 13h 3 days - rep2 |
| GSM436414 |
V. riparia (VR, PI588259) SD, 13h 3 days - rep3 |
| GSM436415 |
V. riparia (VR, PI588259) SD, 13h 7 days - rep1 |
| GSM436416 |
V. riparia (VR, PI588259) SD, 13h 7 days - rep2 |
| GSM436417 |
V. riparia (VR, PI588259) SD, 13h 7 days - rep3 |
| GSM436418 |
V. riparia (VR, PI588259) SD, 13h 14 days - rep1 |
| GSM436419 |
V. riparia (VR, PI588259) SD, 13h 14 days - rep2 |
| GSM436420 |
V. riparia (VR, PI588259) SD, 13h 14 days - rep3 |
| GSM436421 |
V. riparia (VR, PI588259) SD, 13h 21 days - rep1 |
| GSM436422 |
V. riparia (VR, PI588259) SD, 13h 21 days - rep2 |
| GSM436423 |
V. riparia (VR, PI588259) SD, 13h 21 days - rep3 |
| GSM436424 |
V. riparia (VR, PI588259) SD, 13h 28 days - rep1 |
| GSM436425 |
V. riparia (VR, PI588259) SD, 13h 28 days - rep2 |
| GSM436426 |
V. riparia (VR, PI588259) SD, 13h 28 days - rep3 |
| GSM436427 |
V. riparia (VR, PI588259) SD, 13h 42 days - rep1 |
| GSM436428 |
V. riparia (VR, PI588259) SD, 13h 42 days - rep2 |
| GSM436429 |
V. riparia (VR, PI588259) SD, 13h 42 days - rep3 |
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| Relations |
| BioProject |
PRJNA118913 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17502_RAW.tar |
161.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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