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Status |
Public on Apr 29, 2021 |
Title |
Columbia University/NYP COVID-19 Lung Atlas |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We profiled 116,314 cells using snRNA-seq of 20 frozen lungs obtained from 19 COVID-19 decedents and seven control patients with short postmortem interval (PMI) autopsies. The COVID-19 cohort comprises seven female and 12 male decedents, including 13 patients of Hispanic ethnicity, with an age range from 58 to >89 years who had acquired SARS-CoV-2 infection and succumbed to the disease. The average time from symptom onset to death was 27.5 days (range, 4–63 days). After rapid autopsy with a median PMI of 4 hours (range 2–9 hours) collected tissues were either flash-frozen or frozen following OCT (optimal cutting temperature) embedment and subjected to snRNA-seq using a droplet-based platform (10x Genomics). All included patients had underlying hypertensive disorder and frequently one or more additional co-morbidities associated with increased risk for severe COVID-19.
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Overall design |
Single-nuclei RNA sequencing of 116,314 cells from 20 frozen lungs obtained from 19 COVID-19 decedents and seven control patients.
Raw read files to be uploaded to DUOS (https://duos.broadinstitute.org/) due to patient privacy concerns.
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Web link |
https://doi.org/10.1038/s41586-021-03569-1
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Contributor(s) |
Izar B, Melms JC, Biermann J |
Citation(s) |
33915568 |
Submission date |
Apr 05, 2021 |
Last update date |
Sep 20, 2021 |
Contact name |
Jana Biermann |
E-mail(s) |
jb4424@cumc.columbia.edu
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Organization name |
Columbia University
|
Street address |
622 West 168th St
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City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (27)
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Relations |
BioProject |
PRJNA719842 |