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| Status |
Public on Apr 29, 2021 |
| Title |
Columbia University/NYP COVID-19 Lung Atlas |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We profiled 116,314 cells using snRNA-seq of 20 frozen lungs obtained from 19 COVID-19 decedents and seven control patients with short postmortem interval (PMI) autopsies. The COVID-19 cohort comprises seven female and 12 male decedents, including 13 patients of Hispanic ethnicity, with an age range from 58 to >89 years who had acquired SARS-CoV-2 infection and succumbed to the disease. The average time from symptom onset to death was 27.5 days (range, 4–63 days). After rapid autopsy with a median PMI of 4 hours (range 2–9 hours) collected tissues were either flash-frozen or frozen following OCT (optimal cutting temperature) embedment and subjected to snRNA-seq using a droplet-based platform (10x Genomics). All included patients had underlying hypertensive disorder and frequently one or more additional co-morbidities associated with increased risk for severe COVID-19.
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| |
|
| Overall design |
Single-nuclei RNA sequencing of 116,314 cells from 20 frozen lungs obtained from 19 COVID-19 decedents and seven control patients.
Raw read files to be uploaded to DUOS (https://duos.broadinstitute.org/) due to patient privacy concerns.
|
| Web link |
https://doi.org/10.1038/s41586-021-03569-1
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| |
|
| Contributor(s) |
Izar B, Melms JC, Biermann J |
| Citation(s) |
33915568 |
| Submission date |
Apr 05, 2021 |
| Last update date |
Sep 20, 2021 |
| Contact name |
Jana Biermann |
| E-mail(s) |
jb4424@cumc.columbia.edu
|
| Organization name |
Columbia University
|
| Street address |
622 West 168th St
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (27)
|
|
| Relations |
| BioProject |
PRJNA719842 |
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