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Series GSE169390 Query DataSets for GSE169390
Status Public on Sep 25, 2021
Title Single-cell RNA-Seq of premature Rhesus macaques lungs with intra-uterine inflammation and therapuetic blockade
Organism Macaca mulatta
Experiment type Expression profiling by high throughput sequencing
Summary Intrauterine inflammation/infection (IUI), which is present in up to 40% of premature births, leads to elevated levels of pro-inflammatory mediators and microbial products within the amniotic fluid, which come in close contact to fetal mucosae. Yet, knowledge on the fetal mucosal responses to IUI exposure remains limited. To address these questions, we used a non-human primate model of IUI, in which pregnant Rhesus macaques received intra-amniotic (IA) LPS, compared with saline IA. We found that IA LPS exposure induced a robust and rapid inflammation of the fetal lung.
Overall design Single-cell RNA-Seq was performed in the premature Rhesus macaques lung in the presence and absence of LPS. Therapuetic intervention was tested in LPS animals with IL-1R, TNF-alpha or combination blockade.
Contributor(s) Jackson CM, Mukherjee S, Senthamaraikannan P, Presicce P, Kallapur SG, Miller LA, Lewkowich IP, Demmert M, Salomonis N, Jobe AH, Zacharias WJ, Chougnet CA
Citation(s) 35353543
Submission date Mar 22, 2021
Last update date Jul 11, 2022
Contact name Nathan Salomonis
Organization name Cincinnati Children's Hospital
Department Biomedical Informatics
Lab Nathan Salomonis
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platforms (1)
GPL27943 Illumina NovaSeq 6000 (Macaca mulatta)
Samples (13)
GSM5201844 fetal lungs, Saline-1
GSM5201845 fetal lungs, Saline-2
GSM5201846 fetal lungs, LPS-1
BioProject PRJNA716367
SRA SRP311767

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Supplementary file Size Download File type/resource
GSE169390_RAW.tar 285.5 Mb (http)(custom) TAR (of H5)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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