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| Status |
Public on Mar 12, 2021 |
| Title |
The brain transcriptome of the wolf spider, Schizocosa ocreata |
| Organism |
Schizocosa ocreata |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study was to lay the groundwork for comparative transcriptomics of sex differences in the brain of wolf spiders, a non-model organism of the pyhlum Euarthropoda, by generating transcriptomes and analyzing gene expression. To examine differences in sex-differential gene expression, short read transcript sequencing and de novo transcriptome assembly were performed. Messenger RNA (mRNA) was isolated from dissected brain tissue of male and female subadult and mature wolf spiders (Schizocosa ocreata). The data consist of short read sequences for the two different life stages in each sex. Computational analyses on these data include de novo transcriptome assembly, using Trinity and CAP3 assembly suites, and differential expression analysis using the edgeR package. Sample-specific and combined transcriptomes, gene annotations, and differential expression results are described in this data note and are available from associated database submissions.
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| Overall design |
Sequencing and differential expression analyses were performed using dissected brain tissue of male and female immature (subadult) and mature (adult) wolf spiders (Schizocosa ocreata). The analysis was performed with biological replicates of 3 immature males, 2 immature females, 2 mature males, and 3 mature females.
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| Contributor(s) |
Stribling D, Chang PL, Dalton JE, Conow CA, Rosenthal M, Hebets E, Graze RM, Arbeitman MN |
| Citation(s) |
34162407 |
| BioProject |
PRJNA694130 |
| |
| Submission date |
Mar 11, 2021 |
| Last update date |
Jul 02, 2021 |
| Contact name |
Michelle Arbeitman |
| E-mail(s) |
michelle.arbeitman@med.fsu.edu
|
| Phone |
645-9846
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| Organization name |
Florida State University College of Medicine
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| Department |
Biomedical Sciences
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| Lab |
Arbeitman Lab
|
| Street address |
1115 W. Call St
|
| City |
Tallahassee |
| State/province |
FL |
| ZIP/Postal code |
32306 |
| Country |
USA |
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| Platforms (1) |
| GPL29840 |
Illumina HiSeq 2500 (Schizocosa ocreata) |
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| Samples (10)
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| Relations |
| SRA |
SRP302932 |
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