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Series GSE168766 Query DataSets for GSE168766
Status Public on Mar 12, 2021
Title The brain transcriptome of the wolf spider, Schizocosa ocreata
Organism Schizocosa ocreata
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this study was to lay the groundwork for comparative transcriptomics of sex differences in the brain of wolf spiders, a non-model organism of the pyhlum Euarthropoda, by generating transcriptomes and analyzing gene expression. To examine differences in sex-differential gene expression, short read transcript sequencing and de novo transcriptome assembly were performed. Messenger RNA (mRNA) was isolated from dissected brain tissue of male and female subadult and mature wolf spiders (Schizocosa ocreata). The data consist of short read sequences for the two different life stages in each sex. Computational analyses on these data include de novo transcriptome assembly, using Trinity and CAP3 assembly suites, and differential expression analysis using the edgeR package. Sample-specific and combined transcriptomes, gene annotations, and differential expression results are described in this data note and are available from associated database submissions.
Overall design Sequencing and differential expression analyses were performed using dissected brain tissue of male and female immature (subadult) and mature (adult) wolf spiders (Schizocosa ocreata). The analysis was performed with biological replicates of 3 immature males, 2 immature females, 2 mature males, and 3 mature females.
Contributor(s) Stribling D, Chang PL, Dalton JE, Conow CA, Rosenthal M, Hebets E, Graze RM, Arbeitman MN
Citation(s) 34162407
BioProject PRJNA694130
Submission date Mar 11, 2021
Last update date Jul 02, 2021
Contact name Michelle Arbeitman
Phone 645-9846
Organization name Florida State University College of Medicine
Department Biomedical Sciences
Lab Arbeitman Lab
Street address 1115 W. Call St
City Tallahassee
State/province FL
ZIP/Postal code 32306
Country USA
Platforms (1)
GPL29840 Illumina HiSeq 2500 (Schizocosa ocreata)
Samples (10)
GSM5169126 Immature Female Rep 2
GSM5169127 Immature Female Rep 3
GSM5169128 Immature Male Rep 1
SRA SRP302932

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Supplementary file Size Download File type/resource
GSE168766_Detailed_Methods.pdf 134.5 Kb (ftp)(http) PDF
GSE168766_Differential_Expression.xlsx 11.1 Mb (ftp)(http) XLSX
GSE168766_Gene_Annotation.txt.gz 9.7 Mb (ftp)(http) TXT
GSE168766_Gene_Expression_Values.txt.gz 405.7 Kb (ftp)(http) TXT
GSE168766_Transcriptome_Assembly_Statistics.pdf 193.1 Kb (ftp)(http) PDF
GSE168766_Trimmed_Read_Quality_Statistics.pdf 1.2 Mb (ftp)(http) PDF
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Raw data are available in SRA
Processed data are available on Series record

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