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Series GSE165371 Query DataSets for GSE165371
Status Public on Jan 22, 2021
Title A transcriptomic atlas of mouse cerebellar cortex reveals novel cell types
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The cerebellar cortex is a well-studied brain structure with diverse roles in motor learning, coordination, cognition, and autonomic regulation. Nonetheless, a complete inventory of cerebellar cell types is presently lacking. We used high-throughput transcriptional profiling to profile more than 600,000 nuclei and molecularly define cell types across individual lobules of the adult mouse cerebellum. Purkinje neurons showed considerable regional specialization, with the greatest diversity occurring in the posterior lobules. For multiple types of cerebellar interneurons, the molecular variation within each type was more continuous, rather than discrete. For the unipolar brush cells (UBCs)—an interneuron population previously subdivided into discrete populations—the continuous variation in gene expression was associated with a graded continuum of electrophysiological properties. Most surprisingly, we found that molecular layer interneurons (MLIs) were composed of two molecularly and functionally distinct types. Both show a continuum of morphological variation through the thickness of the molecular layer, but electrophysiological recordings revealed marked differences between the two types in spontaneous firing, excitability, and electrical coupling. Together, these findings provide the first comprehensive cellular atlas of the cerebellar cortex, and outline a methodological and conceptual framework for the integration of molecular, morphological, and physiological ontologies for defining brain cell types.
Overall design Characterization and annotation of single nuclei transcriptomes from adult and developing mouse cerebella. Nuclei suspensions for adult mouse cerebellum profiles were generated from 2 female and 4 male adult mice (60 days old), from individual dissections across 16 regions. Nuclei suspensions for developing mouse cerebellum profiles were generated from 2 E18 mice, 2 P0 (newborn) mice, 2 P4 (4 days old) mice, 2 P8, 2 male P12 and 2 male P16 mice.

>>>Raw data are unavailable for human samples due to patient privacy concerns<<<
Contributor(s) Kozareva V, Martin C, Osorno T, Rudolph S, Guo C, Vanderburg C, Nadaf N, Regev A, Regehr W, Macosko E
Citation(s) 34369877, 34620856, 35022615
Submission date Jan 22, 2021
Last update date Mar 04, 2022
Contact name Evan Macosko
Organization name Broad Institute
Street address 75 Ames St, Broad Institute
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (2)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (141)
GSM5032258 Male6_AN1
GSM5032259 Female2_AN1a
GSM5032260 Male2_AN1a
BioProject PRJNA694175
SRA SRP302925

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE165371_cb_adult_mouse.tar.gz 2.9 Gb (ftp)(http) TAR
GSE165371_cb_dev_ints.tar.gz 44.4 Mb (ftp)(http) TAR
GSE165371_cb_processed_initial.tar.gz 4.1 Gb (ftp)(http) TAR
GSE165371_golgi_joint.tar.gz 55.3 Mb (ftp)(http) TAR
GSE165371_granule_joint.tar.gz 1.3 Gb (ftp)(http) TAR
GSE165371_human_combined.tar.gz 1.3 Gb (ftp)(http) TAR
GSE165371_interneuron_joint.tar.gz 453.8 Mb (ftp)(http) TAR
GSE165371_ubc_joint.tar.gz 56.9 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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