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Series GSE16401 Query DataSets for GSE16401
Status Public on Jun 01, 2010
Title tomato salt response
Organism Solanum lycopersicum
Experiment type Expression profiling by array
Summary Wild halophytic tomato has long been considered as an ideal gene donor for improving salt tolerance in tomato cultivars. Here, a wild tomato genotype, Solanum pimpinellifolium ‘PI365967’ is significantly more salt-tolerant than a cultivar, Solanum lycopersicom ‘moneymaker’. Affymetrix Tomato Genome Arrays was used to compare the transcriptome change of PI365967 and Moneymaker by salt treatment.After treatment with 200 mM NaCl for 5 h, PI365967 showed relatively fewer responsive genes compared to Moneymaker. Salt Overly Sensitive (SOS) pathway was found to be more active in PI365967 than in Moneymaker, coinciding with relatively less accumulation of Na+ in shoots of PI365967. A gene encoding salicylic acid-binding protein 2 (SABP2) was induced by salinity only in PI365967, suggesting a possible role of salicylic acid signaling in salt response of PI365967. The fact that two genes encoding lactoylglutathione lyase were salt-inducible only in PI365967, together with much higher basal expression of several glutathione S-transferase genes, suggested a more effective detoxification system in PI365967.
Key words: salt tolerance, transcriptomic profiling, wild tomato, ion homeostasis, SABP2.
Overall design In one treatment, 9 six-leaf-old plants of one tomato genotype (PI365967 or Moneymaker) were divided into three biological replicates and treated hydroponically under sterile condition with (or without) 200 mM NaCl for 5 h. RNA isolated from pooled three individual plants was used in hybridization to one chip, resulting in 12 chips in total.
Contributor(s) Sun W, Xu X, Liu A, Zhu H, Hua X
Citation(s) 20410049
Submission date Jun 03, 2009
Last update date Dec 24, 2015
Contact name Wei Sun
Organization name Institute of Botany, Chinese Academy of Sciences
Street address 20 Nan Xin Cun
City Beijing
ZIP/Postal code 100093
Country China
Platforms (1)
GPL4741 [Tomato] Affymetrix Tomato Genome Array
Samples (12)
GSM412055 tomato Moneymaker unstressed 5 h rep1.
GSM412056 tomato Moneymaker salt stressed 5 h rep1.
GSM412057 tomato PI365967 unstressed 5 h rep1.
BioProject PRJNA116293

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Supplementary file Size Download File type/resource
GSE16401_RAW.tar 12.4 Mb (http)(custom) TAR (of CEL, CHP)
GSE16401_SAM_Moneymaker_5h+_vs._Moneymaker_5h.xls.gz 1.7 Mb (ftp)(http) XLS
GSE16401_SAM_PI365967_5h+_vs._PI365967_5h.xls.gz 1.7 Mb (ftp)(http) XLS
Processed data included within Sample table
Processed data provided as supplementary file

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