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| Status |
Public on Feb 09, 2021 |
| Title |
Diverse CGG binding proteins across eukaryotes produced by independent domestications of hAT transposons |
| Platform organism |
synthetic construct |
| Sample organisms |
Locusta migratoria; Acyrthosiphon pisum; Aedes aegypti; Culex quinquefasciatus; Petromyzon marinus; Latimeria chalumnae; Acipenser ruthenus; Cyprinus carpio; Salmo salar; Fundulus heteroclitus; Sparus aurata; Chrysemys picta; Crocodylus porosus; Gallus gallus; Homo sapiens; Rhodnius prolixus; Erpetoichthys calabaricus; Rhipicephalus appendiculatus; Kryptolebias marmoratus; Nematostella vectensis; Austrofundulus limnaeus; Hucho hucho; Anabas testudineus; Acanthochromis polyacanthus; Amphiprion ocellaris; Labeo rohita; Mola mola; Hippocampus comes; Amphiprion percula; Salarias fasciatus; Sphaeramia orbicularis; Hofstenia miamia; Anabarilius grahami; Rhizophagus irregularis; Paramormyrops kingsleyae |
| Experiment type |
Other
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| Summary |
The human transcription factor (TF) CGGBP1 (“CGG Binding Protein”) is conserved only in amniotes, and is believed to derive from the zf-BED and Hermes transposase DNA-binding domains (DBDs) of a hAT DNA transposon. Here, we examine the DNA binding preferences of a wide variety of metazoan CGGBP1-like TFs with this bipartite domain using PBM experiments. The derived motifs are available at ...
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| Overall design |
Protein binding microarray (PBM) experiments were performed for 106 CGGBP1-like proteins and related hAT transposons. Briefly, the PBMs involved binding GST-tagged DNA-binding proteins to two double-stranded 44K Agilent microarrays, each containing a different DeBruijn sequence design, in order to determine their sequence preferences. Details of the PBM protocol are described in Berger et al., Nature Biotechnology 2006.
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| Contributor(s) |
Yellan I, Yang A |
| Citation(s) |
33561217 |
| Submission date |
Aug 28, 2020 |
| Last update date |
May 11, 2021 |
| Contact name |
Isaac Yellan |
| E-mail(s) |
isaac.yellan@mail.utoronto.ca
|
| Organization name |
University of Toronto
|
| Department |
Molecular Genetics
|
| Street address |
160 College St.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S 3E1 |
| Country |
Canada |
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| Platforms (1) |
| GPL11260 |
Agilent custom ME and HK design array [8mer] |
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| Samples (258)
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| Relations |
| BioProject |
PRJNA660048 |
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