|
| Status |
Public on Aug 24, 2020 |
| Title |
Translational repression of G3BP in cancer and germ cells suppresses stress granules and enhances stress tolerance |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Stress granules (SG) are membrane-less ribonucleoprotein condensates that form in response to various stress stimuli via phase separation. SG act as a protective mechanism to cope with acute stress, but persistent SG have cytotoxic effects that are associated with several age-related diseases. Here, we demonstrate that the testis-specific protein, MAGE-B2, increases cellular stress tolerance by suppressing SG formation through translational inhibition of the key SG nucleator G3BP. MAGE-B2 reduces G3BP protein levels below the critical concentration for phase separation and suppresses SG initiation. Importantly, knockout of the MAGE-B2 mouse ortholog confers hypersensitivity of the male germline to heat stress in vivo. Thus, MAGE-B2 provides cytoprotection to maintain mammalian spermatogenesis, a highly thermo-sensitive process that must be preserved throughout reproductive life. These results demonstrate a mechanism that allows for tissue-specific resistance against stress through fine-tuning phase separation and could aid in the development of male fertility therapies
|
| |
|
| Overall design |
RNA-Seq using Illumina sequencing in WT and KO animals’ whole testes with and without heat stress to characterize how loss of Mageb4 affects the transcriptome
|
| |
|
| Contributor(s) |
Lee A, Klein J, Wang Y, Potts R |
| Citation(s) |
32692974 |
| Submission date |
May 04, 2020 |
| Last update date |
Aug 24, 2020 |
| Contact name |
Yong-Dong Wang |
| E-mail(s) |
michael.wang@stjude.org
|
| Phone |
9015954224
|
| Organization name |
St. Jude Children's Research Hospital
|
| Street address |
262 Danny Thomas Pl, MS1135
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38134 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (21)
|
|
| Relations |
| BioProject |
PRJNA630196 |
| SRA |
SRP259999 |
Less...
More...