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| Status |
Public on Apr 23, 2023 |
| Title |
Long Non-coding RNA NR2F1-AS1 Induces Breast Cancer Dormancy in Lungs by Regulating NR2F1 and ΔNp63 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We show that the epithelial-like and mesenchymal-like subpopulations of breast cancer stem-like cells (BCSCs) demonstrate different levels dormancy and tumorigenicity in lungs. The long non-coding RNA (lncRNA) molecule NR2F1-AS1 (NAS1) is up-regulated in the dormant BCSC subpopulation, and functionally promotes tumor dissemination but reduces proliferation in lungs. Mechanically, NAS1 promotes internal ribosome entry site (IRES)-mediated NR2F1 translation, leading to inhibition of ΔNp63 transcription by NR2F1. Further, ΔNp63 downregulation results in epithelial-mesenchymal transition, reduced tumorigenicity and enhanced dormancy of cancer cells in lungs.
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| Overall design |
Transcriptomic sequencing was firstly performed in MCF10 breast cancer cell line series that include MCF10AT, MCF10CA1h, MCF10CA1a, CA1h-P1 (CD24-CD44med subpopulation of MCF10CA1h) and CA1h-P2 (CD24-CD44high subpopulation of MCF10CA1h), while MCF10CA1h and CA1h-P2 were identified with dormant characteristics. We found that the long non-coding RNA (lncRNA) molecule NR2F1-AS1 (NAS1) is up-regulated in the dormant cells. Then the transcriptomes of CA1h-P2 with NAS1 knockdown, MCF10CA1h and MCF7 with NR2F1 overexpression, and their respective controls were sequenced to find the molecules affected by both NAS1 and NR2F1.
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| Contributor(s) |
Hu G, Liu Y, Zhang P |
| Citation(s) |
34475402 |
| Submission date |
Apr 24, 2020 |
| Last update date |
Jul 24, 2023 |
| Contact name |
Guohong Hu |
| E-mail(s) |
ghhu@sibs.ac.cn
|
| Organization name |
Shanghai Institute of Nutrition and Health
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| Street address |
320 Yueyang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA627846 |
| SRA |
SRP258273 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE149265_gene_CPM_TMM_anno_MCF10_cells.xlsx |
2.9 Mb |
(ftp)(http) |
XLSX |
| GSE149265_gene_CPM_TMM_anno_NAS1_KD.xlsx |
2.2 Mb |
(ftp)(http) |
XLSX |
| GSE149265_gene_CPM_TMM_anno_NR2F1_OE.xlsx |
2.5 Mb |
(ftp)(http) |
XLSX |
| GSE149265_gene_count_mat_MCF10_cells.xlsx |
2.2 Mb |
(ftp)(http) |
XLSX |
| GSE149265_gene_count_mat_NAS1_KD.xlsx |
1.8 Mb |
(ftp)(http) |
XLSX |
| GSE149265_gene_count_mat_NR2F1_OE.xlsx |
2.1 Mb |
(ftp)(http) |
XLSX |
| GSE149265_isoform_CPM_TMM_anno_MCF10_cells.xlsx |
12.2 Mb |
(ftp)(http) |
XLSX |
| GSE149265_isoform_CPM_TMM_anno_NAS1_KD.xlsx |
7.7 Mb |
(ftp)(http) |
XLSX |
| GSE149265_isoform_CPM_TMM_anno_NR2F1_OE.xlsx |
10.4 Mb |
(ftp)(http) |
XLSX |
| GSE149265_isoform_count_mat_MCF10_cells.xlsx |
9.1 Mb |
(ftp)(http) |
XLSX |
| GSE149265_isoform_count_mat_NAS1_KD.xlsx |
6.7 Mb |
(ftp)(http) |
XLSX |
| GSE149265_isoform_count_mat_NR2F1_OE.xlsx |
7.9 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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