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Series GSE148729 Query DataSets for GSE148729
Status Public on May 04, 2020
Title Gene expression profiling of SARS-CoV-1/2 infected human cell lines at bulk and single-cell level
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary To identify human genes and cellular pathways influencing SARS-CoV-1 and SARS-CoV-2 infection, we performed genome-wide measurements of different aspects of gene expression at the bulk and single-cell level.
Overall design Different human cell lines were infected with SARS-CoV-1 and SARS-CoV-2 and harvested at different time points. Gene expression profiling was measured using bulk and single-cell polyA RNA-sequencing, smallRNA sequencing, and totalRNA sequencing.

Raw data are not provided for AEC samples due to patient privacy concerns.
Contributor(s) Wyler E, Mösbauer K, Franke V, Diag A, Gottula LT, Arsie R, Klironomos F, Koppstein D, Ayoub S, Buccitelli C, Richter A, Legnini I, Ivanov A, Mari T, Del Giudice S, Papies JP, Praktiknjo S, Müller MA, Niemeyer D, Selbach M, Akalin A, Rajewsky N, Drosten C, Landthaler M
Citation(s) 33585804
Submission date Apr 15, 2020
Last update date Mar 29, 2021
Contact name Emanuel Wyler
Phone +49 30 9406 3009
Organization name Max Delbrück Center for Molecular Medicine
Department Berlin Institute for Medical Systems Biology
Lab RNA Biology and Posttranscriptional Regulation
Street address Robert Roessle Str 10
City Berlin
ZIP/Postal code 13125
Country Germany
Platforms (3)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (203)
GSM4477854 H1299_polyA_mock-36h-A
GSM4477855 H1299_polyA_mock-36h-B
GSM4477856 H1299_polyA_mock-4h-A
BioProject PRJNA625518
SRA SRP256479

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE148729_AEC_SARSCoV2_17AAG_readcounts.tsv.gz 1.1 Mb (ftp)(http) TSV
GSE148729_Caco2_polyA_readcounts.tsv.gz 1.1 Mb (ftp)(http) TSV
GSE148729_Calu3_polyA_series1_readcounts.tsv.gz 1.2 Mb (ftp)(http) TSV
GSE148729_Calu3_polyA_series2_readcounts_rev.tsv.gz 1.1 Mb (ftp)(http) TSV
GSE148729_Calu3_scRNAseq_morethan1000genes_metadata.tsv.gz 1.1 Mb (ftp)(http) TSV
GSE148729_Calu3_scRNAseq_morethan1000genes_rawcounts_tr.tsv.gz 151.5 Mb (ftp)(http) TSV
GSE148729_Calu3_smallRNA_miRNAcounts.tsv.gz 24.6 Kb (ftp)(http) TSV
GSE148729_Calu3_totalRNA_readcounts.tsv.gz 792.2 Kb (ftp)(http) TSV
GSE148729_H1299_polyA_readcounts.tsv.gz 1.3 Mb (ftp)(http) TSV
GSE148729_H1299_scRNAseq_morethan2000genes_metadata.tsv.gz 593.9 Kb (ftp)(http) TSV
GSE148729_H1299_scRNAseq_morethan2000genes_rawcounts_tr.tsv.gz 108.0 Mb (ftp)(http) TSV
GSE148729_Hsp90_gene_counts.tsv.gz 1.1 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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