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Series GSE133951 Query DataSets for GSE133951
Status Public on Dec 31, 2019
Title Functional specification of CCK+ interneurons by alternative isoforms of Kv4.3 auxiliary subunits
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary CCK-expressing interneurons (CCK+INs) are crucial for controlling hippocampal activity. We found two firing phenotypes of CCK+INs in rat CA3 area; either possessing a previously undetected membrane potential-dependent firing or regular firing phenotype, due to different low-voltage-activated potassium currents. These different excitability properties destine the two types for distinct functions, because the former is essentially silenced during realistic 8-15 Hz oscillations. The general excitability, morphology and gene-profiles of the two types were surprisingly similar. Even the expression of Kv4.3 channels were comparable, despite evidences showing that Kv4.3-mediated currents underlie the distinct firing properties. Instead, the firing phenotypes were correlated with the presence of distinct isoforms of Kv4 auxiliary subunits (KChIP1 vs. KChIP4e and DPP6S). Our results reveal the underlying mechanisms of two previously unknown types of CCK+INs and demonstrate that alternative splicing of few genes, which may be viewed as a minor event in the cells’ whole transcriptome, can underlie distinct cell-type identity.
Overall design mRNA profiles of 17 CCK+/CNR1+ CA3 interneurons, 8 showing rectifying firing (TOR type), 9 showing regular firing (RS type)
Contributor(s) Oláh VJ, Lukacsovich D, Winterer J, Lörincz A, Nusser Z, Földy C, Szabadics J
Citation(s) 32490811
Submission date Jul 08, 2019
Last update date Jun 08, 2020
Contact name Csaba Földy
Organization name University of Zurich
Department Brain Research Institute
Lab Foldy Lab
Street address Winterthurerstrasse 190
City Zurich
State/province Zurich
ZIP/Postal code 8057
Country Switzerland
Platforms (1)
GPL20084 Illumina NextSeq 500 (Rattus norvegicus)
Samples (17)
GSM3930576 C2
GSM3930577 C4
GSM3930578 C5
BioProject PRJNA553202
SRA SRP213647

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Supplementary file Size Download File type/resource
GSE133951_RPKM_gene_counts.tsv.gz 1.2 Mb (ftp)(http) TSV
GSE133951_TPM_gene_counts.tsv.gz 1.3 Mb (ftp)(http) TSV
GSE133951_gene_counts.tsv.gz 343.2 Kb (ftp)(http) TSV
GSE133951_normalized_gene_counts.tsv.gz 943.0 Kb (ftp)(http) TSV
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