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| Status |
Public on Nov 13, 2019 |
| Title |
miRNA expression in Abelson virus transformed fetal liver B cell progenitors |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Knockout of the ubiquitously expressed microRNA-17~92 cluster in mice produces a lethal developmental defect and blocked B lymphopoiesis. We validated the equally widely expressed Bcl2l11 gene as joint target of miR-17~92 cluster members. Bcl2l11 encodes the pro-apoptotic protein BIM, central to life-death decisions in most mammalian cells. To study the contribution of miR-17~92:Bim interaction to miR-17~92 overall function, we set up a system of conditional mutagenesis of the Bim 3’UTR in the mouse. Blocking miR-17~92:Bim interaction early in development phenocopied the lethal developmental defect of miR-17~92 ablation. In contrast, hematopoietic and B lineage specific mutagenesis, while selectively compromising B lineage cell fitness, left hematopoietic cell compartments untouched as long as the cells expressed Bim biallelically. Thus, despite hundreds of overall predicted targets vital miRNA functions can be mediated by a single target gene, depending on cellular context and level of target gene expression.
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| Overall design |
Total RNA was extracted from 5 wild type and 5 Bim3'UTRmut/mut Abelson transformed B cell progenitors derived from E14.5 fetal liver. 100 ng of total RNA per sample was used as input for nCounter gene expression analysis (NanoString Technologies).
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| Contributor(s) |
Rajewsky K, Klironomos F, Labi V |
| Citation(s) |
31699777 |
| Submission date |
Dec 11, 2018 |
| Last update date |
Nov 13, 2019 |
| Contact name |
Verena Labi |
| E-mail(s) |
verena.labi@i-med.ac.at
|
| Organization name |
Medical University Innsbruck
|
| Department |
Developmental Immunology
|
| Street address |
Innrain 80-82
|
| City |
Innsbruck |
| ZIP/Postal code |
6020 |
| Country |
Austria |
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| Platforms (1) |
| GPL23053 |
NanoString nCounter Mouse miRNA Expression Assay |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE123673 |
Vital role and diversity of miRNA control through a single target gene |
|
| Relations |
| BioProject |
PRJNA509484 |
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