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Series GSE122425 Query DataSets for GSE122425
Status Public on Nov 16, 2018
Title Effects of NSUN2 deficiency on the mRNA 5-methylcytosine modification and gene expression profile in HEK293 cells (RNA-Seq)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Aim: To study the biological function of NSUN2 in regulating gene expression and cell proliferation.
Materials & methods: The NSUN2 gene was knocked down in HEK293 cells via CRISPR/Cas9 system. Gene expression were assessed using RNA-Seq.
Results: A total of 790 differentially expressed genes (DEGs) were identified.
Overall design mRNA profiles of wild type (WT) and NSUN2-/- HEK293 cells were generated by deep sequencing, in triplicate.
Contributor(s) Cui H, Sun Z
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Submission date Nov 12, 2018
Last update date Mar 27, 2019
Contact name Zhen Sun
Organization name Yangzhou University
Street address Wenhui
City Yangzhou
ZIP/Postal code 225009
Country China
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM3466389 WT rep1
GSM3466390 WT rep2
GSM3466391 WT rep3
BioProject PRJNA505172
SRA SRP168405

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Supplementary file Size Download File type/resource
GSE122425_Group_293-VS-293-NK_DE_significant_anno.xlsx 66.9 Kb (ftp)(http) XLSX
GSE122425_all.counts.293_vs_293NK.edgeR_all.xls.gz 3.3 Mb (ftp)(http) XLS
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Processed data are available on Series record

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