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| Status |
Public on Aug 14, 2018 |
| Title |
Ehrlichia chaffeensis TRP47 binding sites in human genome |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Ehrlichia chaffeensis is an obligately intracellular bacterium that establishes infection in mononuclear phagocytes through largely undefined reprogramming strategies. Recently, E. chaffeensis effectors Ank200, TRP120, and TRP32 have been shown to function as nucleomodulins that enter the host nucleus and directly modulate transcription of genes implicated in cellular processes such as transcription regulation, apoptosis, phosphorylation, and immune cell activation. In this study, we found that E. chaffeensis TRP47 enters the host cell nucleus and binds regulatory regions of multiple host genes relevant to infection.
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| Overall design |
ChIP-seq was performed using E. chaffensis-infected THP-1 cells at 48 hpi.
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| Contributor(s) |
Kibler CE, McBride JW |
| Citation(s) |
30408047 |
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| Submission date |
Aug 13, 2018 |
| Last update date |
Nov 13, 2018 |
| Contact name |
Clayton Eugene Kibler |
| E-mail(s) |
claykibler@gmail.com
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| Phone |
281-755-6203
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| Organization name |
UTMB
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| Department |
Pathology
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| Lab |
Keiller 1.136
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| Street address |
301 University Blvd
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| City |
Galveston |
| State/province |
TX |
| ZIP/Postal code |
77550 |
| Country |
USA |
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| Platforms (1) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA485704 |
| SRA |
SRP157613 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE118453_RAW.tar |
3.0 Mb |
(http)(custom) |
TAR (of NARROWPEAK) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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