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Series GSE11705 Query DataSets for GSE11705
Status Public on Nov 01, 2008
Title Induction of adaptive changes in the Streptococcus agalactiae transcriptome by human blood
Organism Streptococcus agalactiae
Experiment type Expression profiling by array
Summary In the present investigation, we conducted a bacterial transcriptome dynamics analysis during GBS incubation with human blood. We observed a large modification in the expression of genes involved in transcriptional regulation and in the metabolism of carbohydrates, purins/pyrimidins and inorganic ions, and an up-regulation of genes involved in attachment and plasminogen contact. We also observed differences in the expression of GBS genes in blood at 40° relative to 37°C.
 
Overall design The serotype III GBS reference strain NEM316 was cultivated in Todd Hewitt broth supplemented with 0.2% yeast extract (THY) in 5% CO2 at 37°C until OD reached 0.7. Culture was harvested by centrifugation 8 minutes at 4000 x g at 37°C, then suspended in phosphate-buffered saline (PBS).
Fresh heparinized human blood was obtained from seven healthy voluntary donors (four males and three females) in accordance with a protocol approved by the Institutional Review Board of the Methodist Hospital Research Institute. The blood was maintained at 37°C no more than one hour prior to use. Bacterial suspension was then incubated with the blood of each donor at 37°C and 40°C under slight rotation to avoid sedimentation of blood and bacterial cells (the volume of blood was equivalent to the volume of bacterial culture prior to centrifugation). Samples were removed for microarrays analysis immediately after adding bacteria, and after 30 and 90 minutes of incubation.
Thus, for each of the seven blood donors, we obtained results for five time points: immediately after mixing the bacterium with the blood (time 0), after 30 minutes of contact with blood (time 1) at 37°C and 40°C, and after 90 minutes of contact with blood (time 2) at 37°C and 40°C.
 
Contributor(s) Mereghetti L, Sitkiewicz I, Green NM, Musser JM
Citation(s) 18769548, 19774088
Submission date Jun 06, 2008
Last update date Mar 19, 2012
Contact name Izabela Sitkiewicz
E-mail(s) iza.sitkiewicz@gmail.com
Organization name National Medicines Institute
Department Epidemiology and Clinical Microbiology
Street address Chełmska 30/34
City Warsaw
ZIP/Postal code 00-725
Country Poland
 
Platforms (1)
GPL6923 TMHRI Streptococcus agalactiae 2K array version1
Samples (34)
GSM297233 Expression of S. agalatiae genes in blood at time 0 (donorB)
GSM297236 Expression of S. agalatiae genes in blood after 30 minutes at 37 degrees (donorB)
GSM297237 Expression of S. agalatiae genes in blood after 90 minutes at 37 degrees (donorB)
Relations
BioProject PRJNA106119

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11705_RAW.tar 152.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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