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Series GSE116855 Query DataSets for GSE116855
Status Public on Feb 28, 2019
Title Maintenance of CTCF and transcription factor-mediated interactions from gametes to the early mouse embryo (ChIP-seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Mechanisms by which the mammalian gametes encode epigenetic information that can be transmitted to the progeny are relatively unknown. We find that many sperm promoters in mouse sperm are occupied by phosphorylated forms of RNA polymerase II and the Mediator complex. Analysis of ATAC-seq data suggest that the same promoters are occupied by components of the transcription complex in GV and MII oocytes and preimplantation embryos. Furthermore, distal ATAC-seq sites containing transcription factor motifs are conserved in monkeys and humans. ChIP-seq analyses confirm that transcription factors, including FoxA1 and the androgen and estrogen receptors, occupy distal enhancers. These sites and their interactions with promoters in the gametes are maintained in the early embryo. Additionally, sperm- or oocyte-specific interactions mediated by CTCF/cohesin are only present in the paternal or maternal chromosome, respectively in the zygote and 2-cell stages. These interactions converge in both chromosomes by the 8-cell stage. These results suggest that the mammalian gametes contain a large repertoire of transcription factors that organize a complex pattern of 3D interactions and can be transmitted to the zygote after fertilization.
Overall design Analysis of transcription factors in sperm by ChIP-seq
Contributor(s) Jung YH, Gold HB, Kremsky I, Rowley MJ, Bixler BJ, Punyawai K, Chan AW, Corces V
Citation(s) 31056445
Submission date Jul 10, 2018
Last update date May 30, 2019
Contact name Yoonhee Jung
Phone 4047274250
Organization name Emory university
Department Human Genetics
Lab Corces lab
Street address 615 Michael Street
City Atlanta
State/province Georgia
ZIP/Postal code 30322
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (19)
This SubSeries is part of SuperSeries:
GSE116857 Maintenance of CTCF and transcription factor-mediated interactions from gametes to the early mouse embryo
BioProject PRJNA480447
SRA SRP152940

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Supplementary file Size Download File type/resource
GSE116855_CONT_SPERM_AR.wig.gz 423.5 Mb (ftp)(http) WIG
GSE116855_CONT_SPERM_ERa.wig.gz 395.6 Mb (ftp)(http) WIG
GSE116855_CONT_SPERM_Foxa1.wig.gz 381.0 Mb (ftp)(http) WIG
GSE116855_CONT_SPERM_H2A.Z.wig.gz 69.6 Mb (ftp)(http) WIG
GSE116855_CONT_SPERM_H3.3.wig.gz 44.8 Mb (ftp)(http) WIG
GSE116855_CONT_SPERM_MED12.wig.gz 278.7 Mb (ftp)(http) WIG
GSE116855_CONT_SPERM_RNAPIIS2p.wig.gz 568.3 Mb (ftp)(http) WIG
GSE116855_CONT_SPERM_RNAPIIS5p.wig.gz 390.8 Mb (ftp)(http) WIG
GSE116855_CONT_SPERM_ZNF143.wig.gz 506.3 Mb (ftp)(http) WIG
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