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Series GSE114511 Query DataSets for GSE114511
Status Public on Dec 21, 2018
Title Sequencing of cfDNA derived from the plasma of individuals of different ages
Organism Homo sapiens
Experiment type Other
Summary We provide the first in vivo evidence of global and local chromatin changes in human aging by analyzing cfDNA from the blood of individuals of different age groups. Our results show that nucleosome signals inferred from cfDNA are consistent with the redistribution of heterochromatin observed in cellular senescence and aging in other model systems. It also revealed age and deteriorating health status correlate with a low-level enrichment of signals from cells in the thyroid gland. In addition, we detected an overall nucleosome loss at several genomic locations, such as transcription start and termination sites, 5’UTR of L1HS retrotransposons and dimeric AluY elements with age.
Overall design We profiled cfDNA extracted from a total of 12 individuals across three different ages: 25 year-old (young), 70 year-old (old), healthy 100 year-old (healthy centenarian) samples and unhealthy 100 year-old (unhealthy centenarian)

Please note that each wig pair (*100kb.Fnor.smooth.wig and *10bp.Fnor.smooth.wig) were generated from triplicates (rep1, rep2, rep3) together and are linked to the corresponding *rep1 sample records.
Contributor(s) Teo YV, Neretti N
Citation(s) 30575273
Submission date May 16, 2018
Last update date Mar 20, 2019
Contact name Nicola Neretti
Organization name Brown University
Department Department of Molecular Biology, Cell Biology & Biochemistry
Street address 70 Ship St
City Providence
State/province RI
ZIP/Postal code 02903
Country USA
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (12)
GSM3143257 Young rep1
GSM3143258 Young rep2
GSM3143259 Young rep3
BioProject PRJNA471637
SRA SRP147273

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Supplementary file Size Download File type/resource
GSE114511_RAW.tar 3.1 Gb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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