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| Status |
Public on Apr 25, 2008 |
| Title |
Expression data from Arabidopsis Seed Compartments at the Globular Embryo Stage. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
We collected globular stage seed compartments from 5 or 7-micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments of an Arabidopsis seed containing a globular stage embryo. For the purposes of this study we broke down the seed into 8 capturable compartments: embyro proper, suspensor, micropylar endosperm, peripheral endosperm, chalazal endosperm, chalazal seed coat, general seed coat, and whole seeds.
Keywords: cell type comparison
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| Overall design |
Globular stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 17 samples (Embryo Proper, Micropylar Endosperm, Peripheral Endosperm, General Seed Coat, Chalazal Seed Coat, and Whole Seeds, 2 biological replicates each and Chalazal Endosperm with 3 biological replicates).
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| Contributor(s) |
Stone SL, Pelletier J, Kirkbride RC, Lu XH, Belmonte MF, Fei J, Bui AQ, Goldberg RB, Harada JJ |
| Citation(s) |
23319655, 20385809 |
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| Submission date |
Apr 25, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
John J Harada |
| E-mail(s) |
jjharada@ucdavis.edu
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| Phone |
530-752-0673
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| Organization name |
University of California, Davis
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| Department |
Plant Biology
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| Lab |
Harada
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| Street address |
One Shields Avenue
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| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (17)
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| This SubSeries is part of SuperSeries: |
| GSE12404 |
Expression data from Arabidopsis Seed Compartments at 5 discrete stages of development |
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| Relations |
| BioProject |
PRJNA113823 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11262_RAW.tar |
31.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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