|
| Status |
Public on Mar 15, 2018 |
| Title |
Neural Stem Cell Extracellular Vesicle-Treated Microglia and Transcriptional Response |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Extracellular vesicles (EVs) were isolated from primary cultures of neural stem cells (NSCs) and used to treat microglia.
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| |
|
| Overall design |
EVs were isolated by serial centrifugation from primary NSCs cultured from the subventricular zone of CD1 mice. Cultured microglia were then subjected to control or EV treatments and RNA isolated by Trizol extraction. Library preparation was performed with an NEBNext Ultra RNA Library Preparation Kit according to the manufacturer’s protocol (New England Biolabs). 2 x 150 base pair reads were generated at a depth of 56-84 million reads per sample on an Illumina HiSeq 4000.
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| |
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| Contributor(s) |
Feliciano DM |
| Citation(s) |
29617675 |
| |
| Submission date |
Feb 20, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
David Matthew Feliciano |
| E-mail(s) |
dfelici@clemson.edu
|
| Phone |
8644821296
|
| Organization name |
Clemson University
|
| Department |
Biological Sciences
|
| Lab |
Neurogenesis and Neurogenetics
|
| Street address |
132 Long Hall
|
| City |
Clemson |
| State/province |
South Carolina |
| ZIP/Postal code |
29634-0314 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA434745 |
| SRA |
SRP133182 |
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