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Series GSE109149 Query DataSets for GSE109149
Status Public on Jan 22, 2019
Title Principles and characteristics of the WRKY regulatory network of early MAMP-triggered immunity [ChIP-Seq]
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary During microbe-associated molecular pattern (MAMP)-triggered immunity (MTI) more than 5000 Arabidopsis genes are significantly altered in their expression and the question arises, how such an enormous reprogramming of the transcriptome can be regulated in a safe and robust manner? For the WRKY transcription factors, which are important regulators not only of this defense response, it appears that they act in a complex regulatory sub-network rather than in a linear fashion, which is much more vulnerable to gene function loss either by pathogen-derived effectors or by mutations. In this study we employed RNA-seq, mass spectrometry and ChIP-seq to find evidence for and uncover principles and characteristics of this network.
Overall design For each of two biological replicates we analysed 4 different conditions: plants of WT and wrky18 wrky40 wrky33 triple mutant genotype, each sampled at 0 h (untreated) and 2 h flg22 treatment. For each condition the IP was perfomred with an anti-allWrky antibody and input DNA served as negative control for the peak calling.
Contributor(s) Birkenbihl RP, Somssich IE, Kracher B
Citation(s) 30044528
Submission date Jan 12, 2018
Last update date Jan 24, 2019
Contact name Barbara Kracher
Organization name Max Planck Institute for Plant Breeding Research
Department Plant-Microbe Interactions
Street address Carl-von-Linné-Weg 10
City Cologne
ZIP/Postal code 50829
Country Germany
Platforms (1)
GPL17639 Illumina HiSeq 2500 (Arabidopsis thaliana)
Samples (16)
GSM2933088 wt_0h_input_1
GSM2933089 wt_2h_input_1
GSM2933090 tri_0h_input_1
This SubSeries is part of SuperSeries:
GSE109151 Principles and characteristics of the WRKY regulatory network of early MAMP-triggered immunity
BioProject PRJNA429782
SRA SRP129015

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE109149_RAW.tar 1.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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