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Series GSE108198 Query DataSets for GSE108198
Status Public on Apr 26, 2019
Title Long-term Functional Maintenance of Primary Human Hepatocytes In Vitro
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Functional maintenance of terminally differentiated cells outside the in vivo microenvironment has proved challenging. Current strategies that manipulate cell-cell or cell-matrix connections are difficult to constitute complex regulatory networks for cell function maintenance. Small molecules are easily combined for flexible spatiotemporal modulations, theoretically favorable for synergetic regulation of cell-innate signaling pathways to maintain cell function in vitro. Here, we developed small-molecule cocktails enabling robust maintenance of primary human hepatocytes (PHHs) longer than four weeks, with gene expression profiles, resembling those of freshly isolated PHHs; and prolong-cultured PHHs, for the first time, could maintain drug-metabolizing activities of enzymes accounting for over 80% of drug-oxidation and support hepatitis B virus infection in vitro for over one month. Our study demonstrates that this chemical approach effectively maintains terminally differentiated hepatocytes in vitro, which could be extended to various cell types.
Overall design Total of 29 samples were analyzed, which included primary human hepatocytes (PHHs) cultured in different condition in vitro. To figure out how terminally differentiated cells rapidly lose their function in vitro, two PHHs samples were compared, which included 24h-Cultured hepatocytes and fresh primary human hepatocytes (F-PHHs) [GSM2893923 and GSM2893924]. For comparison of global gene expression of primary human hepatocytes (PHHs) maintained with small molecules or sandwich culture for different time periods, sample3-29 were analyzed [GSM2893935 - GSM2893963][GSM3629857-GSM3629862].
Web link
Contributor(s) Xiang C, Du Y, Meng G, Deng H
Citation(s) 31023926
Submission date Dec 18, 2017
Last update date Apr 29, 2019
Contact name meng gao fan
Phone 86-13426312883
Organization name peking university
Street address yiheyuan road no.5
City beijing
State/province Haidian District/Beijing
ZIP/Postal code 100871
Country China
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (29)
GSM2893923 24h-Cultured hepatocytes
GSM2893924 F-PHHs
GSM2893935 5C_D1.5_B2
BioProject PRJNA423006
SRA SRP127021

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Supplementary file Size Download File type/resource
GSE108198_genes.fpkm_tracking_24h-Cutured_hepatocytes.xlsx 736.0 Kb (ftp)(http) XLSX
GSE108198_update.xlsx 4.1 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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