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| Status |
Public on Apr 01, 2018 |
| Title |
Global transcriptional changes in U87MG glioblastoma cells upon shRNA-mediated TRIM52 knockdown |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
shRNA-mediated ablation of the RING-finger protein TRIM52 from multiple glioblastoma cell lines reduces proliferation and tumorigenesis. To identify gene signatures underlying this phenomenon, transcritional profile of TRIM52 knockdown cells was compared to control cells. Upon TRIM52 ablation, we find 278 differentially regulated genes. Gene ontology analysis reveals that many of the upregulated genes are associated with glycolysis and biosynthetic processes.
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| Overall design |
U87MG glioblastoma cells were stably transduced with doxycycline-inducible shRNA constructs targeting TRIM52 (two different shRNAs) or controls (two different non-targeting shRNAs). Knockdown was induced for five days using 2µg/ml doxycycline. shRNA expressing cells were sorted based on shRNA-coupled GFP expression via flow cytometry. mRNA sequening was performed in duplicate per shRNA cell line.
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| Contributor(s) |
Benke S, Versteeg GA |
| Citation(s) |
29568378 |
| Submission date |
Dec 11, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Stefan Benke |
| E-mail(s) |
stefan.benke@univie.ac.at
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| Organization name |
Max F. Perutz Laboratories, University of Vienna
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| Street address |
Dr. Bohr-Gasse 9
|
| City |
Vienna |
| State/province |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA421947 |
| SRA |
SRP126511 |
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