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Series GSE107932 Query DataSets for GSE107932
Status Public on Apr 01, 2018
Title Global transcriptional changes in U87MG glioblastoma cells upon shRNA-mediated TRIM52 knockdown
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary shRNA-mediated ablation of the RING-finger protein TRIM52 from multiple glioblastoma cell lines reduces proliferation and tumorigenesis. To identify gene signatures underlying this phenomenon, transcritional profile of TRIM52 knockdown cells was compared to control cells. Upon TRIM52 ablation, we find 278 differentially regulated genes. Gene ontology analysis reveals that many of the upregulated genes are associated with glycolysis and biosynthetic processes.
Overall design U87MG glioblastoma cells were stably transduced with doxycycline-inducible shRNA constructs targeting TRIM52 (two different shRNAs) or controls (two different non-targeting shRNAs). Knockdown was induced for five days using 2µg/ml doxycycline. shRNA expressing cells were sorted based on shRNA-coupled GFP expression via flow cytometry. mRNA sequening was performed in duplicate per shRNA cell line.
Contributor(s) Benke S, Versteeg GA
Citation(s) 29568378
Submission date Dec 11, 2017
Last update date May 15, 2019
Contact name Stefan Benke
Organization name Max F. Perutz Laboratories, University of Vienna
Street address Dr. Bohr-Gasse 9
City Vienna
State/province Vienna
ZIP/Postal code 1030
Country Austria
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (8)
GSM2883505 41109: shNT-1_#1
GSM2883506 41114: shNT-2_#1
GSM2883507 41115: shT52-1_#1
BioProject PRJNA421947
SRA SRP126511

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Supplementary file Size Download File type/resource
GSE107932_RAW.tar 830.0 Kb (http)(custom) TAR (of TXT)
GSE107932_processed_file.csv.gz 573.8 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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